| PCR—SSCP研究肝癌原发灶和转移灶中P16^INK4exon2的突变 |
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| 引用本文： | 曹跃琼 王丰. PCR—SSCP研究肝癌原发灶和转移灶中P16^INK4exon2的突变[J]. 复旦学报(自然科学版), 1998, 37(4): 537-541 |
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| 作者姓名： | 曹跃琼 王丰 |
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| 作者单位： | 复旦大学遗传学研究所 |
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| 摘 要： | 用两步ＰＣＲ法,成功地从石蜡包埋组织ＤＮＡ中扩增了Ｐ＾１６ＩＮＸＫ４ｅｘｏｎ２片段;并用单链构象多态分析ＰＣＲ产物的差异。
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| 关 键 词： | 肝癌 原发灶 转移灶 PCR SSCP DNA |
PCR-SSCP Analyzing Mutation in P16~(INK4)exon2 of Idiopathy and Metastasis of Liver Cancer |
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| Cao Yueqiong, Wang Feng, Qiao Shouyi. PCR-SSCP Analyzing Mutation in P16~(INK4)exon2 of Idiopathy and Metastasis of Liver Cancer[J]. Journal of Fudan University(Natural Science), 1998, 37(4): 537-541 |
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| Authors: | Cao Yueqiong Wang Feng Qiao Shouyi |
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| Abstract: | The P16INK4exon2 has been successfully amplifed by two-step-PCR from tissues embeded in parraffin, and the differences of the PCR products were analyzed by SSCP. Six deletions detected by PCR and one SSCP-detectable mutation were found in 32 idiopathy liver cancers while eleven deletions detected by PCR and two SSCP-detectable mutations in 48 liver cancers. |
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| Keywords: | liver cancers idiopathy metastasis P16~(INK4)exon2 1 PCR SSCP |
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