| 原儿茶酸与拉米夫定体外联合抗HBV药效学研究 |
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| 引用本文： | 吴文华,张晓雷,韩凤梅.原儿茶酸与拉米夫定体外联合抗HBV药效学研究[J].湖北大学学报(自然科学版),2011,33(2):193-196. |
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| 作者姓名： | 吴文华 张晓雷 韩凤梅 |
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| 作者单位： | 湖北大学中药生物技术湖北省重点实验室,湖北武汉,430062 |
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| 基金项目： | 科技部重大专项，湖北省发改委生物产业专项，武汉市科技局攻关项目，武汉大学病毒学国家重点实验室开放研究基金 |
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| 摘 要： | 为观察拉米夫定(3TC)与原儿茶酸(PA)联合应用抗乙型肝炎病毒(HBV)的作用,在HepG2.2.15细胞培养液中分别加入适当浓度的3TC、PA、3TC+ PA溶液,每4d换用含相同药物浓度的新鲜培养液,第8天末取上清液,用酶联免疫吸附测定法(ELISA)检测上清液中HbsAg和HbeAg的量,用荧光定量PCR( F...
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| 关 键 词： | 原儿茶酸 拉米夫定 抗乙型肝炎病毒 HepG2.2.15细胞 |
Pharmacodynamic study of lamivudine combined with protocatechuic acid against hepatitis B virus in vitro |
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| WU Wenhua,ZHANG Xiaolei,HAN Fengmei.Pharmacodynamic study of lamivudine combined with protocatechuic acid against hepatitis B virus in vitro[J].Journal of Hubei University(Natural Science Edition),2011,33(2):193-196. |
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| Authors: | WU Wenhua ZHANG Xiaolei HAN Fengmei |
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| Institution: | (Key Lab of Biotechnology of TCM of Hubei Province,Hubei University,Wuhan 430062,China) |
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| Abstract: | To evaluate the inhibitory action of lamivudine（3TC） combined with protocatechuic acid（PA） against hepatitis B virus in vitro,HepG2.2.15 cell was cultured with diffenrent concentration of 3TC,PA,3TC＋PA,respectively.The culture fluid of HepG2.2.15 cell was replaced with the fresh culture medium containing the same content of the drug every 4 d.At the 8th day,HbsAg,HbeAg and HBV DNA in the culture media were detected.Jin Zhengjun Method was applied to evaluate the drug-drug interaction of 3TC and PA against HBV.The results indicated that the combination group of 3TC and PA had an additive inhibitory action on HbeAg and HBV DNA,a synergistic inhibitory action on HbsAg,respectively when the mass concentration ratio of PA versus 3TC was ≥100. |
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| Keywords: | protocatechuic acid lamivudine anti-HBV HepG2 2 15 cell |
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