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| 噬菌体展示蛋白质芯片的无标记检测 | |
| 引用本文： | 刘芳芳,罗昭锋,定翔,朱圣庚,余兴龙.噬菌体展示蛋白质芯片的无标记检测[J].清华大学学报(自然科学版),2008,48(11). |
| 作者姓名： | 刘芳芳 罗昭锋 定翔 朱圣庚 余兴龙 |
| 作者单位： | 1. 清华大学,精密仪器与机械学系,精密测试技术与仪器国家重点实验室,北京,100084 2. 中国科学技术大学,生命科学实验中心,合肥,230027 3. 北京大学,生命科学学院,北京,100871 |
| 基金项目： | 国家自然科学基金 ， 高等学校博士学科点专项科研基金 |
| 摘 要： | 为获得丰富的特异识别分子和实现无标记、高灵敏度检测,利用噬菌体展示技术,选择在pⅢ蛋白上展示12肽的噬菌体M13作探针,采用羧基-氨基共价耦联法制备噬菌体展示蛋白质芯片.在此基础上,与表面等离子体共振传感技术结合,使用Biacore3000仪器,检测噬菌体展示蛋白质芯片与特异性抗体的反应,测定反应动力学常数.实验结果表明:当抗体浓度为0.4 μmol·L-1时,响应信号可达365±8个单位;不同浓度的抗体与芯片反应的数据较好地与S型曲线吻合.该方法可望用于噬菌体展示蛋白质芯片的检测. |
| 关 键 词： | 表面等离子体共振 噬菌体M13展示系统 噬菌体展示蛋白质芯片 特异性抗体 动力学常数 |
Label-free detection of phage-displayed protein chips |
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| LIU Fangfang,LUO Zhaofeng,DING Xiang,ZHU Shenggeng,YU Xinglong.Label-free detection of phage-displayed protein chips[J].Journal of Tsinghua University(Science and Technology),2008,48(11). | |
| Authors: | LIU Fangfang LUO Zhaofeng DING Xiang ZHU Shenggeng YU Xinglong |
| Institution: | LIU Fangfang1,LUO Zhaofeng2,DING Xiang1,ZHU Shenggeng3,YU Xinglong1(1.State Key Laboratory of Precision Measurement Technology , Instruments,Department of Precision Instruments , Mechanology,Tsinghua University,Beijing 100084,China,2.School of Life Sciences,University of Science , Technology of China,Hefei 230027,3.College of Life Sciences,Peking University,Beijing 100871,China) |
| Abstract: | Surface plasmon resonance and the phage display technique were combined to enable use of a variety of specific reagents for label-free,high sensitivity detection in protein chips.Phage M13 which displays 12 peptides on its coated protein pIII was selected as the probe to prepare the phage-displayed protein chip using the amine coupling method.The specific interactions between the chip and the antibodies were detected using Biacore3000,with the binding kinetic parameters determined.Tests show that an antibod... |
| Keywords: | surface plasmon resonance phage M13 display system phage-displayed protein chip specific antibody kinetic parameter |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |