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| 赤栀黄颗粒多种活性成分的提取优化及动物药效学评价 | |
| 引用本文： | 汤浩,闵光涛,蒋生祥,李玉民.赤栀黄颗粒多种活性成分的提取优化及动物药效学评价[J].兰州大学学报(自然科学版),2008,44(5). |
| 作者姓名： | 汤浩 闵光涛 蒋生祥 李玉民 |
| 作者单位： | 1. 中国科学院,兰州化学物理研究所,甘肃,兰州,730000;中国科学院,研究生院,北京,100039 2. 兰州大学,第一医院普通外科,甘肃,兰州,730000 3. 中国科学院,兰州化学物理研究所,甘肃,兰州,730000 |
| 摘 要： | 通过正交实验设计,对赤栀黄颗粒的多种活性成分进行提取优化,并以动物药效学方法评价优化结果.HPLC同时测定赤栀黄颗粒中栀子苷、芍药苷、大黄酸和大黄素的含量,考察提取时间、提取.溶剂、提取次数对成分提取的影响;以异硫氰酸苯酯造大鼠肝损伤模型,测定血清肝脏酶和胆红素,进行药效学评价.活性成分经水提取3次,每次20 min,可有效阻止异硫氰酸苯酯引起的大鼠肝脏酶和胆红素的升高.经动物药效学试验验证,此活性成分的提取优化条件,有利地保证了赤栀黄颗粒临床疗效的发挥. |
| 关 键 词： | 赤栀黄颗粒 多种活性成分 正交实验 提取优化 肝损伤 动物药效学评价 |
Extractive optimization of multi-active components in Chizhihuang granules and its animal pharmacodynamic evaluation |
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| TANG Hao,MIN Guang-tao,JIANG Sheng-xiang,LI Yu-min.Extractive optimization of multi-active components in Chizhihuang granules and its animal pharmacodynamic evaluation[J].Journal of Lanzhou University(Natural Science),2008,44(5). | |
| Authors: | TANG Hao MIN Guang-tao JIANG Sheng-xiang LI Yu-min |
| Institution: | TANG Hao~(1,2),MIN Guang-tao~3,JIANG Sheng-xiang~1,LI Yu-min~3 (1.Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China,2.Graduate School of Chinese Academy of Sciences,Beijing 100039,3.Department of General Surgery,The First Hospital of Lanzhou University,China) |
| Abstract: | An orthogonal test was designed to optimize the extractive condition of multi-active components in Chizhihuang granules,and the optima's animal pharmacodynamic evaluation was also carried out.Geni- poside,paeoniflorin,rhein,and emodin were simultaneously determined by HPLC to evaluate the influence of the extractive time,extractive solvent,and extractive frequency on the extractive technology;the levels of serum AST,ALT,ALP,and BIL in rat with PITC intoxicated were determined for the pharmaco-dynamic evalua... |
| Keywords: | Chizhihuang granule multi-active component orthogonal test extract technology liver injury animal pharmacodynamic evaluation |
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