| 珙桐休眠期种子高质量基因组DNA的提取及分析 |
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| 引用本文： | 杨艳,苏智先,黎云祥,冯晓,陈光登.珙桐休眠期种子高质量基因组DNA的提取及分析[J].西华师范大学学报(哲学社会科学版),2008,29(4). |
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| 作者姓名： | 杨艳 苏智先 黎云祥 冯晓 陈光登 |
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| 作者单位： | [1]西华师范大学生命科学学院,四川南充637002 [2]四川省高校环境科学与生物多样性保护重点实验室,四川南充637002 [3]四川省生态学与环境保护技术重点实验室,四川绵阳621000 |
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| 基金项目： | 国家自然科学基金资助项目(30670209); |
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| 摘 要： | 在对传统CTAB法进行改进的基础上，提取了沙藏珙桐休眠期去中果皮种子高质量基因组DNA，琼脂糖电泳及紫外分光光度分析，表明其OD260nm／OD280nm在1．8—2．0之间，用所提取的基因组DNA作为模板，S17为引物，进行RAPD扩增，获得了清晰的扩增图谱．
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| 关 键 词： | 珙桐 基因组DNA 提取 检测 |
High Quality Extraction and Analysis of Genome DNA from the Seeds of Davidia Involucrata in Dormant Period |
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| YANG Yan,SU Zhi-xian,LI Yun-xiang,FENG Xiao,CHEN Guang-deng.High Quality Extraction and Analysis of Genome DNA from the Seeds of Davidia Involucrata in Dormant Period[J].Journal of China West Normal University:Natural Science Edition,2008,29(4). |
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| Authors: | YANG Yan SU Zhi-xian LI Yun-xiang FENG Xiao CHEN Guang-deng |
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| Institution: | YANG Yan1,2,SU Zhi-xian3,LI Yun-xiang1,FENG Xiao1,CHEN Guang-deng2(1.College of Life Sciences,China West Normal University,Nanchong 637002,China,2.Key Laboratory of Environmental Science , Biodiversity Conservation of Sichuan Higher Education,3.Sichuan Provincial Key Laboratory of Ecology , Environmental Technology,Mianyang 621000,China) |
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| Abstract: | Based on improving the traditional CTAB method,the high quality genome DNA was extracted from Davidia involucrata seed in dormant period.It indicated that the ratio of OD value at 260nm and 280nm was between 1.8 and 2.0 by agarose gel electrophoresis and UV spectrophotometry.Then,the extracted genome DNA was amplified by RAPD which used S17 as primer and the clear amplification patterns were obtained. |
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| Keywords: | Davidia involucrata genome DNA extraction detection |
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