| 显微外科技术在小鼠器官移植中的应用与体会 |
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| 引用本文： | 陈辉,金昌海,郑翔,鱼达.显微外科技术在小鼠器官移植中的应用与体会[J].实验动物科学,2010,27(6):69-72. |
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| 作者姓名： | 陈辉 金昌海 郑翔 鱼达 |
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| 作者单位： | 浙江大学医学院附属第一医院卫生部多器官联合移植研究重点实验室,浙江省器官移植研究重点实验室,杭州310003 |
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| 基金项目： | 本文受浙江省科技厅重大专项资助 |
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| 摘 要： | 探讨交流显微外科技术在小鼠心脏和肝脏移植中的应用。探讨掌握显微外科技术的要点,并根据文献和自身的经验讨论小鼠动静脉在显微镜下的缝合,以及采用双人"双袖套"法进行门静脉、肝下下腔静脉吻合,支架法重建胆管连接的经验和体会。为器官移植实验技术操作提供参考。
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| 关 键 词： | 显微外科 实验动物 器官移植 |
Application and Understanding of Microsurgical Technique in Mice and Rats Organ Transplantation |
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| CHEN Hui,JIN Chang-hai,ZHENG Xiang,YU Da.Application and Understanding of Microsurgical Technique in Mice and Rats Organ Transplantation[J].Shiyan Dongwu Kexue,2010,27(6):69-72. |
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| Authors: | CHEN Hui JIN Chang-hai ZHENG Xiang YU Da |
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| Institution: | (Key Laboratory of Multi-Organ Transplantation,Ministry of Health;First Affiliated Hospital,Zhejiang University School of Medicine,Key Laboratory of Organ Transplantation,Zhejiang Provence,Hangzhou 310003,China) |
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| Abstract: | Objective To approach the application of microsurgery technique in the heart and liver transplantation of mice.To discuss the highlight of microsurgery technique including： suture the blood vessel of mouse under the microscopy,anastomosis the portal vein and hypohepatic inferior vena cava using double-cuff technique and reconstitute bile duct using frame according to reference and personal experience.The article was in order to provide details of the steps,which is worth noting for organ transplantation experiment. |
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| Keywords: | Microsurgery Laboratory animal Organ transplantation |
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