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| H-2基因检测方法在北京市实验动物遗传质量抽检中的应用 | |
| 引用本文： | 马丽颖,岳秉飞,刘双环. H-2基因检测方法在北京市实验动物遗传质量抽检中的应用[J]. 实验动物科学, 2010, 27(6): 6-8 |
| 作者姓名： | 马丽颖 岳秉飞 刘双环 |
| 作者单位： | 中国药品生物制品检定所,北京100050 |
| 摘 要： | 目的为贯彻实施新国标,通过检测小鼠H-2单倍型,确认近交系小鼠免疫遗传质量。方法依据新国标GB/T14927.2-2008中的微量细胞毒检测方法,检测北京地区主要生产厂家的BALB/c和C57BL/6小鼠共70只的H-2单倍型。结果共抽检4个单位的BALB/c和C57BL/6近交系小鼠70只,经检测BALB/c为H-2Dd和H-2Kd,C57BL/6为H-2Db和H-2Kb,符合率100%,质量符合要求。结论经抽检,北京地区主要生产厂家2个品系小鼠免疫遗传质量合格。 |
| 关 键 词： | 实验动物 遗传 检测 |
H-2 Gene Detection Method Apply for Beijing Laboratory Animal Genetic Quality |
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| MA Li-ying,YUE Bing-fei,LIU Shuang-huan. H-2 Gene Detection Method Apply for Beijing Laboratory Animal Genetic Quality[J]. Laboratory Animal Science, 2010, 27(6): 6-8 | |
| Authors: | MA Li-ying YUE Bing-fei LIU Shuang-huan |
| Affiliation: | (The National institute of the Control of Pharmaceutical and Biological Products,Beijing 100050,China) |
| Abstract: | Objective To carry out newly GB and examine the genetic characteristics of the inbred strain mouse.Methods Accorded to the new methods of microcytotoxicity test in GB/T14927.2-2008,checked H-2 haploid of Beijing area manufacturer＇s mainly BALB/c and C57BL/6 inbred strain mouse.Results All 4 units together 70 inbred strain mouse BALB/c haploid is H-2Dd and H-2Kd,C57BL/6 haploid is H-2Db and H-2Kb,percent of pass 100%.Conclusion According to sample Beijing area produces mainly immune hereditary 2 strain inbred mouse are qualified. |
| Keywords: | Laboratory animal Heredity Monitoring |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |