稻瘟病菌粗毒素对水稻幼胚愈伤组织的影响及抗性筛选

利用目前稻瘟病不同的优势小种菌株的液体培养提取的粗毒素对水稻幼胚进行处理,稻瘟病菌粗毒素提取液对水稻幼胚的愈伤的诱导和分化的有明显的抑制效应。随着毒素浓度的增加,水稻胚性愈伤获得率和分化能力显著降低。在诱导培养基中加入毒素对幼胚的筛选取效果较好,可以获得较多的胚性愈伤和较多的分化苗。培养基中毒素的浓度处理以30%效果好。水稻幼胚的抗性筛选过程中胚性愈伤获得和分化苗数较高,是很好的培养材料;而且不同的水稻品种产生的耐性不同,并可以同时筛选出抗稻瘟病的突变体。

The Infection of Young Embryo Callus by the Crude Toxin of Phyricularis oryzae .cav and Blast-resistant Mutant-screening

The young embryo induction,ca llus subculture and differentiation were strong.Inhibited by the crude toxin filtration,Contrasted with CK,the results were significant different,and the result was different with toxin concentration changing.With the increasing of the toxin concentration and the prolonging the impact time,the inhibiting was stronger.30% was the better toxin concentration when screening of rice blast-resistant mutant from the yong mature embryo culture;Contrasted the methods of screening of rice blast-resistant mutant,using the media including toxin to inducting mutant was better.