麻疯树核糖体失活蛋白curcin基因的原核表达及其抗真菌活性的研究

通过PCR方法,将麻疯树核糖体失活蛋白curcin的开放阅读框片段从麻疯树总DNA中扩增出来.并将该克隆基因片段连接到原核表达载体pQE-30中,成功构建了重组质粒pQE-C,转化大肠杆菌M15菌株.经IPTG诱导后,curcin重组蛋白在大肠杆菌中成功表达.然后通过纯化原核表达产物,得到纯化的curcin重组蛋白,并以纸片法进行体外抗菌活性检测,发现其具有较强抗真菌活性.

Prokaryotic expressed ribosome-inactivating protein (curcin) with anti-fungal activity from Jatropha curcas L.

The open reading frame (ORF)of curcin was amplified from total DNA of Jatropha curcas by PCR method. The cloned gene fragments were ligated into a prokaryotic expression vector pQE-30 and the recombination plasmid pQE-C was constructed. Then the pQE-C was transformed into E. coli M15, and the recombination curcin was expressed in M15 under IPTG induction. The purified recombination curcin was obtained and its antibacterial activity was tested in vitro by paper disk method. It was found that curcin had a better antifungal activity.