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水稻Xa7基因抗白叶枯病应答中的活性氧代谢分析
引用本文:马伯军,郑茜茜,张萍华,胡娜娜,陈析丰. 水稻Xa7基因抗白叶枯病应答中的活性氧代谢分析[J]. 浙江师范大学学报(自然科学版), 2013, 36(1): 1-5
作者姓名:马伯军  郑茜茜  张萍华  胡娜娜  陈析丰
作者单位:浙江师范大学化学与生命科学学院,浙江金华,321004
基金项目:国家重大科技专项,国家自然科学基金资助项目,浙江省自然科学基金资助项目,浙江省重点科技创新团队项目,浙江省公益性技术应用研究计划项目
摘    要:
白叶枯病是由革兰氏阴性黄单孢菌水稻变种(Xanthomonas oryzae pv.Oryzae,Xoo)所引起的一种世界性水稻细菌病害.水稻Xa7基因是一个具有广谱抗性的显性抗白叶枯病基因.通过对水稻抗病品种IRBB7(含Xa7)和感病对照IR24接种白叶枯菌PX086,发现:在叶片的病原菌侵染部位,IRBB7比IR24的活性氧(H2O2和O2-)积累更快且含量更高;与活性氧代谢相关的酶,如超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶和过氧化物酶的活性也更高.推测活性氧的代谢调节可能在Xa7基因介导的抗病反应中起作用.

关 键 词:水稻  抗白叶枯病  Xa7基因  活性氧

Metabolism of reactive oxygen species in resistant response of rice Xa7 gene to bacterial blight
MA Bojun , ZHENG Xixi , Zhang Pinghua , HU Na'na , CHEN Xifeng. Metabolism of reactive oxygen species in resistant response of rice Xa7 gene to bacterial blight[J]. Journal of Zhejiang Normal University Natural Sciences, 2013, 36(1): 1-5
Authors:MA Bojun    ZHENG Xixi    Zhang Pinghua    HU Na'na    CHEN Xifeng
Affiliation:( College of Chemistry and Life Science, Zhejiang Normal University, Jinhua Zhejiang 321004, China)
Abstract:
Bacterial blight ( BB ) , caused by Xanthomonas oryzae pv. Oryzae, is one of the most serious worldwide disease of rice. Xa7 is a broadly dominant resistance gene directed against BB. The resistant rice cultivar IRBB7 ( with Xa7 ) and susceptible control IR24 were inoculated with strain PXO86 of BB. Comparison to the IR24, the leaves of IRBB7 accumulated reactive oxygen species (ROS; H2O2 and 02 - ) faster and higher, the activity of active oxygen metabolism-related enzymes ( SOD, CAT, APX and POD ) of IRBB7 was higher in the infect site. It was suggested that the regulation of ROS metabolism might be helpful for the Xa7- mediated disease resistance response.
Keywords:rice  resistance of bacterial blight  Xa7 gene  reactive oxygen species
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