昆布多糖对氯化镉诱导斑马鱼骨骼发育毒性的改善作用

探讨不同浓度的昆布多糖对氯化镉诱导的斑马鱼骨骼发育毒性的改善作用。选用发育正常的72 hpf斑马鱼胚胎，加入100 μg/L浓度的氯化镉，同时加入不同浓度（25、50、100 μg/L）的昆布多糖。3 d后，用钙黄绿素进行染色2 h，在体视荧光显微镜下观察斑马鱼骨骼发育的情况，拍照并计算硬骨部分的荧光染色面积。结果显示，不同浓度的昆布多糖溶液对氯化镉诱导的骨骼发育毒性均呈现出一定的改善作用，且呈剂量依赖关系，100 μg/L浓度的昆布多糖溶液对斑马鱼骨骼发育的改善作用最显著，25 μg/L浓度作用最弱。研究结果表明，昆布多糖对氯化镉诱导的斑马鱼骨骼发育毒性具有明显的改善作用。

Recovery effect of laminarin on cadmium chloride induced developmental toxicity of zebrafish skeleton

We addressed recovery effect of different concentrations of laminarin on developmental toxicity of zebrafish skeleton induced by cadmium chloride. We selected 72 hpf normal growth zebrafish embryos, and added 100 μg/L of cadmium chloride and 25, 50, 100 μg/L of laminarin. Three days later, they were dyed for 2 h by calcein. We observed skeletal growth of zebrafish on microscope, and calculated the fluorescent staining area of hard bone. Results show that different concentrations of laminarin can all reduce the toxicity of zebrafish skeletal growth induced by cadmium chloride, and dose dependent relation exists. Laminarin of 100 μg/L has significant recovery effect, but the effect is the worst for laminarin of 25 μg/L. This shows that laminarin has significant recovery effect on cadmium chloride induced zebrafish skeletal developmental toxicity.