| 茅莓黄酮液相色谱-电喷雾质谱研究 |
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| 引用本文： | 汪瑗,张荻生,才华. 茅莓黄酮液相色谱-电喷雾质谱研究[J]. 北京教育学院学报(自然科学版), 2006, 0(3) |
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| 作者姓名： | 汪瑗 张荻生 才华 |
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| 作者单位： | 首都师范大学化学系 北京100037 |
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| 摘 要： | 采用紫外光谱定性定量分析野生植物茅莓主要有效成分黄酮化合物,并对其进行液相色谱-电喷雾质谱联用。液相色谱流动相A:CH3OH、B:H2O 1%乙酸,电喷雾负离子质谱(-ESI)。液相色谱流出时间16.21min的化合物为六羟基双氢黄酮醇二聚体形式,分子量640,占总黄酮的91.71%;流出时间19.61min的化合物分子量448,为槲皮甙,占总黄酮的8.29%。
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| 关 键 词： | 茅莓 黄酮 液相色谱-电喷雾质谱 |
Study of Flavones in Rubus Parvifolius Linn by High Performance Liquid Chromatography-Electrospray Ionization-mass |
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| Wang Yuan Zhang Disheng Cai Hua. Study of Flavones in Rubus Parvifolius Linn by High Performance Liquid Chromatography-Electrospray Ionization-mass[J]. Journal of Beijing Institute of Education(Natural Science Edition), 2006, 0(3) |
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| Authors: | Wang Yuan Zhang Disheng Cai Hua |
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| Abstract: | Qualitative and quantitative analyses for total flavones in wild Rubus parvifolius Linn (RPL)were performed using Ultraviolet Spectroscopy and isolation and structure determination of their main active ingredients have been carried out by high performance liquid chromatography(HPLC),thin layer chromatography(TLC),coupled with Electrospray ionization-mass Spectroscopy(ESI-MS).Two flavones in RPL were separated,and they are herahydroxy dihydroflavanonol and quercetin. Two chromatographic spots were gotten when polyamide TLC plate was used to separate the flavones,Two compounds were separated and identified by HPLC-ESI-MS. The constructions of compound 1 tR 16.21min,dimer -ESI m/z639, in order to 3, 5, 7, 3', 4', 5'-Hexahydroxy dihydroflavanonol monomer m/z319. The constructions of compound 2 tR 19.61min, -ESI m/z 301, in order to quercitroside. |
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| Keywords: | Rubus parvifolius Linn tea HPLC-ESI-MS flavones |
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