吡咯伯克霍尔德氏菌JK-SH007分批发酵 动力学模型的构建

吡咯伯克霍尔德氏菌JK-SH007是分离自杨树中的一种细菌,其对杨树病原真菌具有抑制作用,是一种具有生防应用潜力的有益菌株。为深入了解该菌株的发酵进程,放大发酵规模,并对发酵过程进行最优化控制,笔者对JK-SH007菌株液体分批发酵产生抑菌活性的动力学进行了研究。根据发酵实验数据,分别选用Logistic 方程、Luedeking-Piret 方程和Luedeking-Piret like方程建立了模拟菌体生长、抑制真菌活性产生和葡萄糖消耗的动力学模型。经拟合分析发现,动力学模型的计算值与实验值拟合良好,可直观反映JK-SH007菌株分批发酵过程中的动力学特征。

Construction of batch fermentation kinetics model of Burkholderia pyrrocinia strain JK-SH007

Burkholderia pyrrocinia JK-SH007 isolated from poplar can inhibit the pathogenic fungi in poplar. To optimum fermentation process of strain JK-SH007, fermentation kinetics were investigated in this study. A kinetic model for the simulation of bacterial growth, the inhibition of fungal activity generation and glucose consumption were constructed by using Logistic equation, Luedeking-Piret equation and Luedeking-Piret like equation, respectively, based on the data from fermentation experiment. The calculated values in kinetic models fit well with experimental values which directly reflected the kinetic characteristics during the batch fermentation process of strain JK-SH007. Moreover, the fermentation kinetics model provided a foundation for the optimization and amplification of fermentation process.