| SARS冠状病毒核衣壳蛋白cDNA的克隆和原核表达载体的构建 |
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| 引用本文： | 安丽萍,王笑英,曾常茜,杜培革,金莹.SARS冠状病毒核衣壳蛋白cDNA的克隆和原核表达载体的构建[J].北华大学学报(自然科学版),2004,5(4):312-315. |
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| 作者姓名： | 安丽萍 王笑英 曾常茜 杜培革 金莹 |
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| 作者单位： | 北华大学,医学院,吉林,吉林,132013;吉林省人民医院,吉林,长春,130000 |
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| 摘 要： | 目的克隆SARS冠状病毒核衣壳(N)蛋白.方法用RT-PCR技术克隆SARS冠状病毒N蛋白全长cDNA,cDNA经序列分析鉴定后,克隆到pET30a表达载体.结果实现了SARS冠状病毒N蛋白基因的克隆.结论可以对重组成功的pET30a-N进行进一步的应用.
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| 关 键 词： | SARS冠状病毒 N蛋白 克隆 |
| 文章编号： | 1009-4822(2004)04-0312-04 |
| 修稿时间： | 2004年4月21日 |
Cloning and Constructing of Prokaryotic Expression Vector of SARS Coronavirus Nucleocapsid Protein cDNA |
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| AN Li-ping,WANG Xiao-ying,ZENG Chang-qian,DU Pei-ge,JIN Ying.Cloning and Constructing of Prokaryotic Expression Vector of SARS Coronavirus Nucleocapsid Protein cDNA[J].Journal of Beihua University(Natural Science),2004,5(4):312-315. |
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| Authors: | AN Li-ping WANG Xiao-ying ZENG Chang-qian DU Pei-ge JIN Ying |
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| Institution: | AN Li-ping~1,WANG Xiao-ying~2,ZENG Chang-qian~1,DU Pei-ge~1,JIN Ying~2 |
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| Abstract: | ObjectiveTo clone nucleocapsid protein from SARS coronavirus.MethodsAccording to published SARS coronavirus genome sequences, the full length cDNA of N protein from SARS coronavirus was cloned by RT-PCR and the cDNA was cloned into the pET30a expression vector.ResultsProkaryotic N protein of SARS coronavirus was obtained.ConclusionThe pET30a-N can be used for further functional study of SARS coronavirus N protein. |
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| Keywords: | SARS coronavirus Nucleocapsid protein Cloning |
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