| L-甲硫氨酸高产菌株的紫外线诱变育种 |
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| 引用本文： | 马燕,黄敏,黄春萍.L-甲硫氨酸高产菌株的紫外线诱变育种[J].四川大学学报(自然科学版),2004,41(4):861-864. |
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| 作者姓名： | 马燕 黄敏 黄春萍 |
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| 作者单位： | 1. 四川抗菌素工业研究所,成都,610051
2. 四川师范大学生命科学学院,成都,610066 |
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| 摘 要： | 以一株具有M-海因水解酶系统产L-甲硫氨酸(L-Met)的芽孢杆菌MV0073为出发菌株，用含亮氨酸平板定向选育，经过6轮紫外诱变，筛选出系列L-Met高产突变株，其中菌株M604的L-Met产量最高．在相同培养条件下，M604的L-Met产量是出发菌株的3．14倍，且L-Met能以较高含量持续到发酵结束．M604连续传代6次，遗传性状比较稳定．
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| 关 键 词： | L-甲硫氨酸 M-海因酶 紫外诱变 |
| 文章编号： | 0490-6756(2004)04-0861-04 |
Breeding by Ultraviolet Ray Mutation of L-methionine Producing Strain |
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| MA Yan ,HUANG Min ,HUANG Chun-ping ,HUANG Min.Breeding by Ultraviolet Ray Mutation of L-methionine Producing Strain[J].Journal of Sichuan University (Natural Science Edition),2004,41(4):861-864. |
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| Authors: | MA Yan HUANG Min HUANG Chun-ping HUANG Min |
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| Institution: | MA Yan 1,HUANG Min 2,HUANG Chun-ping 2,HUANG Min 2 |
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| Abstract: | UV mutagenesis was performed on Bacillus MV0073 which has L-5-thiomethyhydantoinase system. The mutants were selected directionally on the agar plate containing Leucine,and a series of new L-methionine-overproducing mutants was obtained by six UV mutagenesis One of the mutants M604 at the same cultured condition,the production of L-methionine by the strain was 3 14 times of that by original strain.The mutant M604 was generated for six times and showed that the L-methionine-overproducing capacity was stable. |
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| Keywords: | L-methionine L-5-thiomethylhy-dantoinase ultraviolet ray mutation |
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