姜黄素诱导肝癌HepG2细胞凋亡及其对bcl-2及survivin蛋白表达的影响

目的 研究姜黄素对HepG2细胞凋亡的促进作用,并探讨其可能的的作用机制.方法 实验分为空白对照组、1 mg/ml、1.5mg/ml、2mg/ml的姜黄素处理组,MTT法检测不同浓度姜黄素对细胞增殖抑制情况；电镜观察细胞形态及其结构变化；流式细胞术检测凋亡率；Western blot检测各组作用24 h后细胞中bcl-...

Curcumin Induces Cell Apoptosis and Its Effect on bcl-2 and survivin Expression in HepG2 Cells

Objective The study was to observe the effect of Curcumin inducing apoptosis in human hepatocellular carcinoma HepG2 cells, and to investigate its possible mechanism. Methods Experiments were divided into negative control group, 1 mg/ml curcumin group, 1.5 mg/ml curcumin group,2 mg/ml curcumin group. MTT assay was used to determine the proliferation inhibition of HepG2 ceils. The morphological changes of cells were observed by transmission microscope. The cell apoptosis was detected by flow cytometry （FCM）. Western blot analysis was used to examine the expression of bcl-2 and survivin protein in each group after treated 24 h. Results A concentrations of Curcumin had a certain degree of growth inhibition on HepG2 ceils, and showed a dose- dependent and time-dependent manner. Apoptotic body could be observed under transmission microscope. FCM analysis demonstrated that the apoptosis rates of in each group were （4. 85 ± 0. 31 ） %, （ 8.25 ± 0.46 ） %, （ 11.44 ±0. 56 ） %, and （ 17.68±0. 98 ） % respectively. Western blot analysis demonstrated that the expression level of bcl-2 and survivin protein decreased with the increasing of Curcumin concentration. Conclusion Curcumin significantly inhibited the proliferation and induced cell apoptosis in HepG2 cells, and its mechanism may through down-regulating the expression of bel-2 and survivin.