| 正常和细胞质雄性不育水稻线粒体DNA限制性内切酶的酶切图谱分析 |
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| 引用本文： | 卓德艮,叶正祥,汪训明.正常和细胞质雄性不育水稻线粒体DNA限制性内切酶的酶切图谱分析[J].复旦学报(自然科学版),1988(4). |
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| 作者姓名： | 卓德艮 叶正祥 汪训明 |
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| 作者单位： | 湖南省农科院，复旦大学遗传学研究所，复旦大学遗传学研究所 研究生 |
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| 摘 要： | 从N,cms-WA,cms-BT和cms-RL四种不同细胞质源的水稻中制备线粒体DNA。用限制性内切酶Pst Ⅰ,Hind Ⅲ和Xho Ⅰ等酶切线粒体DNA经琼脂糖凝胶电泳,发现水稻N,cms-WA,cms-BT和cms-RL细胞质确有不同的线粒体DNA,不同的保持系之间也存着一定的差异。水稻线粒体DNA最小分子的大小在195～245kb之间。
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| 关 键 词： | 水稻 细胞质雄性不育 线粒体DNA 限制性内切酶。 |
RESTRICTION ENDONUCLEASE ANALYSIS OF MITOCHONDRIAL DNA FROM NORMAL AND THE CYTOPLASMIC MALE-STERILE RICE |
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| Zhuo Degen,Ye Zhengxiang,Wang Xunming,.RESTRICTION ENDONUCLEASE ANALYSIS OF MITOCHONDRIAL DNA FROM NORMAL AND THE CYTOPLASMIC MALE-STERILE RICE[J].Journal of Fudan University(Natural Science),1988(4). |
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| Authors: | Zhuo Degen Ye Zhengxiang Wang Xunming |
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| Institution: | Institute of Genetics |
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| Abstract: | The rice mitochondrlal DNAs (mt-DNAs) were prepared from members of N(fertile), cms-WA, cms-BT and cms-RL groups. Restriction endonucleases Pst I,Hind III and Xho I were used to digest the mt-DNAs, and the fragments resultedwere run the gel-electrophoresis and compared. The results showed that they werequite distinct from each other and that mitochondrial DNAs from the NormalZhenxian 97 B and Nonghu 26 B Were different. The estimated minimum molecularsizes of rice mt-DNAs ranged from 195~245kb. |
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| Keywords: | rice cytoplasmic male-sterility mitochondrial DNA (mt-DNA) restriction endonuclease |
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