| 用二氧化碳电极测定霉菌537蛋白酶的活性 |
| |
| 引用本文： | 王也如,匡育勤.用二氧化碳电极测定霉菌537蛋白酶的活性[J].复旦学报(自然科学版),1994,33(4):468-472. |
| |
| 作者姓名： | 王也如 匡育勤 |
| |
| 作者单位： | 复旦大学，上海新型酶制剂厂，复旦大学化学系 |
| |
| 摘 要： | 在霉菌５３７蛋白酶的作用下，酷蛋白发生水解，生成酪氨酸和谷氨酸等组成该蛋白的氨基酸，加入Ｌ－谷氨酸脱羧酶使谷氨酸发生脱羧反应，会释放出ＣＯ２，后者可用ＣＯ２电极进行测定，从而可间接地算出５３７蛋白酶的活性，在蛋白酶活性为２０００－１０００单位范围内，电位的变化值与活性呈现良好的线性关系。
|
| 关 键 词： | 二氧化碳 电极 蛋白酸 霉菌 |
Determination of mould 537 proteinase activity using pCO_2 electrode |
| |
| Wang Yeru,Deng Jiaqi.Determination of mould 537 proteinase activity using pCO_2 electrode[J].Journal of Fudan University(Natural Science),1994,33(4):468-472. |
| |
| Authors: | Wang Yeru Deng Jiaqi |
| |
| Institution: | Wang Yeru;Deng Jiaqi(Fudan University)Kuang Yuqin(Shanghai New Type Enzyme Factory) |
| |
| Abstract: | nder action of the mould 537 proteinase casein will hydrolyze into its amino acid components,like tyrosine, glutamic acid and so on.Adding of L-glutamate decarboxylase will make the decarboxy reaction of glutamic acid take place, releasing CO2.The CO2 produced can be determined with pCO2 electrode,and the activity of mould 537 proteinase can be calculatd inderectly. The decrease of potential-activity shows good linear relationship within 2 000 to 10 000 unit of proteinase activity in working solution. |
| |
| Keywords: | protelytic enzymes decarboxylation glutamic acid electrochemical sensors carbondioxide electrodes |
| 本文献已被 CNKI 维普 等数据库收录！ |
