| 应用正交实验研究铜绿假单胞菌原生质体制备与再生 |
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| 引用本文： | WEI Ming-bao,张甲耀,王海,方呈祥,崔长征.应用正交实验研究铜绿假单胞菌原生质体制备与再生[J].信阳师范学院学报(自然科学版),2005,18(2):169-171,175. |
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| 作者姓名： | WEI Ming-bao 张甲耀 王海 方呈祥 崔长征 |
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| 作者单位： | 1. 武汉大学,资源与环境科学学院,湖北,武汉,430079
2. 武汉大学,生命科学学院,湖北,武汉,430072 |
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| 基金项目： | 国家自然科学基金资助项目(20277029) |
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| 摘 要： | 通过对铜绿假单胞菌原生质体制备与再生进行正交实验,探讨各因子对铜绿假单胞菌原生质体制备与再生影响水平,得到铜绿假单胞菌原生质体制备与再生的最优条件:酶解时间 0. 5h,酶浓度 2mg/L,菌龄为 15h 此时原生质体形成率为 80. 5%,再生率为 20. 6%
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| 关 键 词： | 正交实验 铜绿假单胞菌 原生质体 |
| 文章编号： | 1003-0972(2005)02-0169-03 |
Study on protoplast formation and regeneration of Pseudomonas aeruginosa with cross-test experiments |
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| WEI Ming-bao,ZHANG Jia-yao,WANG Hai,FANG Cheng-xiang,CUI Chang-zheng.Study on protoplast formation and regeneration of Pseudomonas aeruginosa with cross-test experiments[J].Journal of Xinyang Teachers College(Natural Science Edition),2005,18(2):169-171,175. |
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| Authors: | WEI Ming-bao ZHANG Jia-yao WANG Hai FANG Cheng-xiang CUI Chang-zheng |
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| Institution: | WEI Ming-bao~ |
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| Abstract: | Through on cross-test experiments of protoplast formation and regeneration of Pseudomonas aeruginosa, the affects factors on protoplast formation and regeneration of P. aeruginosa were studied.The optimum conditions are as follows,0.5 hour of the treating time 2 mg/L of the concentration of lysozyme and 15 hours of incubation time. Under the optimum conditions, the ratio of formation and regeneration protoplast are 80.5% and 20.6%,respectively. |
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| Keywords: | cross-test experiment pseudomonas aeruginosa protoplast |
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