| 质粒Yp7构建的探讨 |
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| 引用本文： | 陈升,罗忠训.质粒Yp7构建的探讨[J].湖北大学学报(自然科学版),1993,15(1):20-23. |
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| 作者姓名： | 陈升 罗忠训 |
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| 作者单位： | 湖北大学生物系
(陈升，罗忠训)，湖北大学生物系(胡学俊) |
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| 摘 要： | 以大肠杆菌酵母穿梭质粒YRp7 DNA为材料,经体外重组构建了质粒Yp7。该质粒具有Amp~r、Tet~r两个抗药性标记和一个来自酵母细胞的Trpl基因。分子量约为5.25kb。该质粒可用于筛选水稻等真核生物DNA中的自主复制序列。
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| 关 键 词： | 穿梭质粒 自主复制序列 DNA 质粒 |
THE PROBE OF THE CONSTRUCTION OF PLASMID VECTOR Yp7 |
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| Chen Sheng Luo Zhongxun Hu Xuejun.THE PROBE OF THE CONSTRUCTION OF PLASMID VECTOR Yp7[J].Journal of Hubei University(Natural Science Edition),1993,15(1):20-23. |
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| Authors: | Chen Sheng Luo Zhongxun Hu Xuejun |
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| Institution: | Department of Biology |
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| Abstract: | A plasmid vector Vp7 has been constructed using the shuttle vector YRp7 digested with Bgl E and partially digested with EcoR I . The Yp7 contains Amp\Tet',and a Trpl gene from Succfiu-rtnnyces cerevisiM. Its M. W. is about 5. 25kb. Cloning of a DNA segment in the RamH I site can be easily detected by the loss of Tet'. It's very useful for the cloning and expression of the autonomously repli-acting sequence from the eukaryotic DNA such as rice DNA. |
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| Keywords: | Shuttle vector Autonomously replicating sequence DNA Recombination |
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