BALB/c突变无毛小鼠免疫学特性指标的研究

BALB c突变无毛小鼠的突变基因是位于小鼠第 11号染色体上 ,定位结果表明该突变为一种新的影响小鼠皮肤和被毛结构的突变 ,被命名Uncv(Uncovered) ,已得到小鼠遗传国际命名委员会的认可 ,并已被小鼠基因组资料库收录。皮肤是机体最大的器官 ,也是机体与外界的主要生理屏障 ,它具有产生、维持局部免疫应答和炎症反应的能力 ,许多免疫应答都发生于皮肤。小鼠皮肤和被毛结构的突变是否影响机体的免疫功能 ,解剖观察和饲养繁殖情况表明该小鼠免疫器官健全 ,在普通饲养环境中能够繁殖。为了进一步研究BALB c突变无毛小鼠的突变基因免疫功能 ,…

Study on the Parameters of Specific Immune of BALB/c-Mutant Hairless Mice

The mutant gene of BALB/c-mutant hairless mice was assigned to chromosome 11, Genetic markers which have been tested suggested that the mutation is a new genetic locus that affected the skin and hair structure of the mouse The mutation was named uncoved, with the symbol Uncv . Uncv has been accepted by International Naming Committee of Mice, and the gene information of Uncv has been receipted by the mice's genebank . The skin is the biggest organ of the body , and is also the main physiological barrier between vivo and vitro . The skin is albe to produce and keep the part immunoresponses , inflammation . Many immunoresponses are related to the skin . The dissecting and breeding of the mutant mice show that their immune organs are normal and they can adopt to the general conditions. Objective To further study the mutant gene' s immune function of the BALB/c-mutant hairless mice . Method We tested the parameters of the immune system about two-day-old、two-month-old hairless mice and mutant sparse coat mice . After testing the parameters of CD4 + 、 CD3 + 、 CD8 + 、 CD19 + through flow cytometry and testing the IgG by ELISA. Results we found that CD19+ 、CD4+/CD8+ of male are higher than female about two-week-old mutant mice with sparse coat , but CD8+ of female is higher than the male, CD4+/CD8+ of male F2 sparse coat mice is higher than the female, CD19+ 、IgG of male mutant hairless mice is higher than the female . There were no significant differences among the male and the female about the two-month-old mutant hairless mice and the mutant sparse coat mice, CD4 + of male F2 two-month-old mutant hairless mice is higher than the female . Otherwise , we also found that the celluar immunity and humoral immunity of both two-week-old and two-month-old hairless mice are lower than the mutant sparse coat mice; only the celluar immunity of F2 mutant hairless mice is lower than the mutant sparse coat mice , but the humoral immunity is higher . The experiment suggest that the mutation of mutant hairless mice can down regulate the mice's immunity . Our studies showed that the mutant gene of BALB/c hairless mice affected the immune function.