DNA指纹图及RAPD技术在比格犬群体遗传检测中的应用

比格犬因体型小 ,性情温顺 ,抗病力强 ,生理生化指标均衡而成为生物医学领域公认的理想实验用犬。我国于 2 0世纪 80年代初引入比格犬 ,并已成功地饲养繁育 ,国内形成了广东、北京和上海等主要的比格犬繁育基地。然而在 2 0余年的饲养繁殖和应用研究中 ,有关比格犬的群体遗传研究工作鲜有报道 ,对于比格犬遗传稳定性、基因频率均衡性和比格犬遗传隔离及分化的研究以及种质保护工作也没有深入开展。Falk&Holsinger(1991)指出 :我们不可能保护好我们所不了解的。为有效保护比格犬种质资源 ,防止实验用比格犬近交衰退以及种群的隔离和分化 ,本…

DNA Fingerprinting and RAPD for Genetic Monitoring of Laboratory Beagle

With small somatotype, gentle temperament, balanced hematological and blood biochemical index, Beagle was ideal for laboratory use. Since 1980, beagle has been introducted into our country. They were successfully bred and multiplied in Guangdong , Shanghai and Beijing . But the control of heredity, balance of gene frequency, gene diversity and gene differentiation and protection of idioplasm has not been well studied. Falk & Holsinger( 1991 )point out: we can not protect what were unknowed. For operative preventing the inbreeding diminution , gene segregation and differentiation,the DFP and randomly amplified polymorphic (RAPD) technique were applied to assess the genetic variation and colony heredity structural analysis among the population of three internal beagle centers and Marshell beagle in America. The experimental exponent of the heredity analysis in different colony were from Guangdong, Shanghai, Beijing and Marshell (Amereca), and they were not relative. The experimental exponent of the different generation were from Beijing colony,and they were direct maternal side in different generation, but each individual were no relative in the same generation.