| 球孢白僵菌毒素对昆虫体外培养细胞胞内同功酶和代谢水平的影响 |
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| 引用本文： | 武艺,黄秀梨,邓继先.球孢白僵菌毒素对昆虫体外培养细胞胞内同功酶和代谢水平的影响[J].北京师范大学学报(自然科学版),1997(1). |
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| 作者姓名： | 武艺 黄秀梨 邓继先 |
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| 作者单位： | 北京师范大学生物学系!北京，100875，北京师范大学生物学系!北京，100875，军事医学科学院生物工程研究所!北京，100071 |
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| 摘 要： | 用吸附法从球孢白僵菌（Beauveriabassiana）的代谢产物中提取毒素，并将毒素作用于草地夜蛾（Spodopterafrugiperda）体外培养细胞，以研究球孢白僵菌的昆虫致病机理．同功酶特异性染色结果表明，细胞中毒后，细胞内的乳酸脱氢酶、苹果酸脱氢酶、酯酶等同功酶在种类和含量上都有所减少．并发现其细胞代谢紊乱，当毒素质量浓度在0～500mg·L(－1)时，培养液中的葡萄糖和蛋白的质量浓度均随细胞中毒加深而升高．
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| 关 键 词： | 球孢白僵菌 毒素 体外培养细胞 同功酶 细胞代谢 |
THE EFFECT OF BEAUVERIA BASSIANA TO THEULTRASTRUCTURE AND THE TOTAL OF PROTEIN |
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| Wu Yi, Huang Xiuli, Deng Jixian.THE EFFECT OF BEAUVERIA BASSIANA TO THEULTRASTRUCTURE AND THE TOTAL OF PROTEIN[J].Journal of Beijing Normal University(Natural Science),1997(1). |
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| Authors: | Wu Yi Huang Xiuli Deng Jixian |
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| Abstract: | Beauvericin is isolated from the metabolites of Beauveria bassiana using absorbingmethod, then it is applied to cultured cells of spodoptera frugiperda in vitro. In order to studythe insectcidemechanism of Beauveria bassiana. The result of spedfic isoenzyme dying indicatesthe amount and quantity of LDH, MDH, EST isoenzymes is reduced. The metabolism ofCells is disordered. When the toxin is between 0 - 500 mg. L-1, the concentration of glucoseand protein in medium increase in panel with extent of toxity. |
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| Keywords: | Beauveria bassiana toxin cultured cells in vitro isoenzyme metabolism |
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