Depletion of calcium stores contributes to progesterone-induced attenuation of calcium signaling of G protein-coupled receptors |
| |
Authors: | Katja Gehrig-Burger Jirina Slaninova Gerald Gimpl |
| |
Institution: | (1) Institute of Pharmaceutics and Biochemistry, University of Mainz, Becherweg 30, 55099 Mainz, Germany;(2) Institute of Organic Chemistry and Biochemistry (IOCB), Academy of Sciences of the Czech Republic (ASCR), Prague, Czech Republic |
| |
Abstract: | Progesterone non-genomically attenuates the calcium signaling of the human oxytocin receptor and several other Gαq protein-coupled receptors. High progesterone concentrations are found in the endometrium during pregnancy opposing the responsiveness
of the underlying myometrium to labor-inducing hormones. Here, we demonstrate that within minutes, progesterone inhibits oxytocin-
and bradykinin-induced contractions of rat uteri, calcium responses induced by platelet-activating factor in the human endometrial
cell line MFE-280, and oxytocin-induced calcium signals in PHM1-31 immortalized pregnant human myometrial cells. Using human
embryonic kidney (HEK293) cells as model system, we analyzed the molecular mechanisms underlying these effects. Our data indicate
that progesterone rapidly depletes intracellular calcium stores. The resulting desensitization of the cells might contribute
to the quiescence of the uterus during pregnancy. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|