| 犬瘟热病毒N蛋白抗原表位基因的合成和杆状病毒表达载体的构建 |
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| 引用本文： | 陈旖,刘一,隋丽华,张小飞,崔晓霞,赵彦斌,刘冰,王冬平,孙兆增,曾林.犬瘟热病毒N蛋白抗原表位基因的合成和杆状病毒表达载体的构建[J].实验动物科学,2012,29(4):5-8. |
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| 作者姓名： | 陈旖 刘一 隋丽华 张小飞 崔晓霞 赵彦斌 刘冰 王冬平 孙兆增 曾林 |
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| 作者单位： | 1. 军事医学科学院实验动物中心,北京,100071
2. 军事医学科学院实验动物中心,北京 100071;青岛农业大学动物科技学院,青岛 266109 |
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| 摘 要： | 目的串联合成犬瘟热病毒N蛋白抗原表位基因,并构建其杆状病毒表达载体。方法利用生物信息学软件,预测N蛋白的抗原表位,串联合成这些抗原表位。利用定向克隆的方法,将该基因片段连接到杆状病毒表达载体pFastBac-Hta。结果获得了包含犬瘟热病毒N蛋白抗原表位的基因,并成功构建pFastBac-N合成表达载体。结论该实验为犬瘟热病毒N蛋白抗原表位基因在杆状病毒内的表达奠定了基础。
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| 关 键 词： | 犬瘟热病毒 抗原表位 N蛋白 杆状病毒表达 |
Synthesis the Gene Including the N Protein Antigen Epitopes of CDV and Construction the Baculovirus Expressive Vector |
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| CHEN Yi
,
LIU Yi
,
SUI Li-hua
,
ZHANG Xiao-fei
,
CUI Xiao-xia
,
ZHAO Yan-bin
,
LIU Bing
,
WANG Dong-ping
,
SUN Zhao-zeng
,
ZENG Lin.Synthesis the Gene Including the N Protein Antigen Epitopes of CDV and Construction the Baculovirus Expressive Vector[J].Shiyan Dongwu Kexue,2012,29(4):5-8. |
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| Authors: | CHEN Yi LIU Yi SUI Li-hua ZHANG Xiao-fei CUI Xiao-xia ZHAO Yan-bin LIU Bing WANG Dong-ping SUN Zhao-zeng ZENG Lin |
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| Institution: | 1 (1.Laboratory Animal Center,Academy of Military Medicine Sciences,Beijing,100071,China)(2.College of Animal Science and Technology Qingdao Agricultural University,266109,China) |
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| Abstract: | Objective To synthesize the gene including the CDV-N protein antigen epitopes,and construct the baculovirus expression vector.Methods The N protein antigen epitopes was predicted by bioinformatics software and synthesized tandem.The fragment was cloned into the baculovirus expression vector pFastBac-Hta.Results The gene including the CDV-N protein antigen epitopes was obtained and successfully constructed the pFastBac-Nsynthesis expression vector.Conclusion The experiment laid the foundation for the CDV-N protein antigen epitopes expression in baculovirus. |
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| Keywords: | CDV antigen epitopes N protein Baculovirus expression system |
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