应用HPLC色谱技术进行柃属植物化学分类学研究

利用高效液相色谱(HPLC)技术对24种1变种柃属植物28份样品的化学成分进行检测,并以各样品中色谱峰的相对保留时间对柃属植物的特征化学成分进行标记,最终统计到23个特征色谱峰用于化学分类研究。结果显示,柃属植物色谱峰在种内稳定出现,在种间则呈现多样性,可用于物种的鉴别。为进一步探讨种间亲缘关系及部分种的具体分类学地位,研究同时还采用UPGMA法和NJ法对色谱峰进行聚类分析,并根据两种聚类树型图讨论了化学成分的分类学意义。研究结果表明HPLC色谱技术具有快捷性、直观性和重现好的特点,可作为一种理想的标记用于柃属植物的分类鉴定。此外,本研究还能为柃属进一步的资源利用研究提供重要的参考。

Chemotaxonomy of Eurya by HPLC Chromatograms

Chemotaxonomic study of 25 taxa(24 species and 1 variety) of Eurya, in a total of 28 samples were carried with high performance liquid chromatograms method. Twenty-three chemical components from the whole samples were recognized according to the relative retention time of the chromatograms. The results showed that the chromatograms stabilized between same species and varied among different species, which could be used to distinguish species from each other. In order to reveal the inter-specific relationships and the taxonomic positions of these species, chromatograms data were submitted to cluster analysis using UPGMA and NJ methods and then the taxonomic significance of chemical component in genus Eurya were discussed based on the two dendrograms. Our study showed that the HPLC chromatograms method was rapid, intuitive and repeatable, and it could be mentioned as a good marker to classify the plants of Eurya. In addition, this study also provided useful data for studying the resource utilization in the genus.