模拟微重力大鼠T淋巴细胞发育异常的初步研究

对模拟空间微重力条件下大鼠T细胞发育受阻的神经免疫调节机理进行初步探究.采用流式细胞检测技术测定模拟微重力大鼠胸腺中不同发育阶段T淋巴细胞的数量变化,用ELISA法测定胸腺组织中去甲肾上腺素(NE)和内源性糖皮质激素(GC)的含量.结果表明模拟微重力模型大鼠出现明显胸腺萎缩(p<0.05),CD3⁺CD4⁺CD8^-和CD3⁺CD4^-CD8⁺T细胞数量明显减少(p<0.05);模拟微重力大鼠胸腺中NE和GC含量有升高的趋势.模拟微重力大鼠胸腺中发生了从双阳性T细胞向单阳性T细胞的发育阻滞,这种异常可能与神经内分泌免疫调控相关.

Pilot Study of the T Cell Development in Rat Thymus Under Simulated Microgravity

By using the tail suspension rat model, the effects of the simulated microgravity on T cell development in thymus and the Neuroimmunologic mechanism were explored. The changes of different subtype T lymphocytes in thymus of the tail suspension rat were tested by flow cytometry detection. The content of norepinephrine (NE) and endogenous glucocorticoid (GC) in rat thymus tissue were detected by ELISA tests. Obvious atrophy of thymus organ could be found in tail suspension rats (p<0.05). The data of flow cytometry analysis show that the CD3⁺CD4⁺CD8^- and CD3⁺CD4^-CD8⁺ T cell count decreased significantly in tail suspension rats thymus compared with control group (p<0.05). The ELISA assay showes that the content of NE and GC under simulated microgravity has a tendency to rise. The blocking of CD3⁺CD4⁺CD8⁺ T cells develops to CD3⁺CD4⁺CD8^- and CD3⁺CD4^-CD8⁺ single positive T cells in tail suspension rat thymus, which might associate with neuroendocrine regulation.