| 聚苯乙烯为致孔剂的分子印迹膜对溶菌酶吸附性能的研究 |
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| 引用本文： | 赵文涛,陈振贺,杨戍,张鑫,孙立权,罗爱芹.聚苯乙烯为致孔剂的分子印迹膜对溶菌酶吸附性能的研究[J].北京理工大学学报,2015,35(S1):6-10. |
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| 作者姓名： | 赵文涛 陈振贺 杨戍 张鑫 孙立权 罗爱芹 |
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| 作者单位： | 北京理工大学 生命学院, 北京 100081,北京市环境保护科学研究院 环境分析测试中心, 北京 100037,北京理工大学 生命学院, 北京 100081,北京理工大学 生命学院, 北京 100081,北京理工大学 生命学院, 北京 100081,北京理工大学 生命学院, 北京 100081 |
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| 摘 要： | 探索聚苯乙烯微球的添加对于分子印迹膜对溶菌酶(Lysozyme, Lyz)吸附性能的影响.以溶菌酶为模板分子,丙烯酰胺、N,N'-亚甲基双丙烯酰胺为功能单体、聚苯乙烯(Polystyrene,PS)微球为致孔剂,制备聚苯乙烯为致孔剂的分子印迹聚合物膜(PS-MIP).PS-MIP的吸附平衡时间为30 min,短于未添加聚苯乙烯微球的分子印迹聚合物膜(MIP);PS-MIP与MIP对于Lyz的吸附能力均明显好于聚苯乙烯为致孔剂的非分子印迹聚合物膜(PS-NIP)与未添加聚苯乙烯微球的非分子印迹聚合物膜(NIP);PS-MIP对Lyz的选择性要好于MIP,而卵清蛋白和牛血清白蛋白在PS-MIP和MIP上的荧光信号没有差别.实验结果表明,在分子印迹膜聚合过程中添加聚苯乙烯微球,有利于缩短吸附时间,增大溶菌酶的吸附量并提升选择性.
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| 关 键 词： | 溶菌酶 分子印迹聚合物膜 聚苯乙烯 荧光检测 |
| 收稿时间： | 2015/3/20 0:00:00 |
Characterization of Molecularly Imprinted Membrane Modified by Polystyrene Microspheres as Porogen for Lysozyme Adsorption |
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| ZHAO Wen-tao,CHEN Zhen-he,YANG Shu,ZHANG Xin,SUN Li-quan and LUO Ai-qin.Characterization of Molecularly Imprinted Membrane Modified by Polystyrene Microspheres as Porogen for Lysozyme Adsorption[J].Journal of Beijing Institute of Technology(Natural Science Edition),2015,35(S1):6-10. |
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| Authors: | ZHAO Wen-tao CHEN Zhen-he YANG Shu ZHANG Xin SUN Li-quan and LUO Ai-qin |
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| Institution: | School of Life Science, Beijing Institute of Technology, Beijing 100081, China,Municipal Research Institute of Environmental Protection, Beijing 100037, China,School of Life Science, Beijing Institute of Technology, Beijing 100081, China,School of Life Science, Beijing Institute of Technology, Beijing 100081, China,School of Life Science, Beijing Institute of Technology, Beijing 100081, China and School of Life Science, Beijing Institute of Technology, Beijing 100081, China |
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| Abstract: | A study on the membrane components of rat hippocampus was presented. The isoelectric focusing OFFGEL combing with the reversed-phase liquid chromatography and mass spectrometry was adopted as the major tool to study the separation and enrichment capability of tryptic peptides. The database for annotation, visualization and integrated discovery(DAVID) was used to analyze the acidic proteins we separated based on GO (gene ontology). The results indicate that there are 24 different fractions, each of which is identified by nano-reversed-phase liquid chromatography coupled with quadruple tandem time-of-flight mass spectrophotometer (Q-TOF). About 77% of the identified peptides are found in only one fraction. Moreover, little deviations are observed at low pH value and it has a good focusing effect. |
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| Keywords: | lysozyme molecularly imprinted polymer membranes polystyrene fluorescence detection |
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