| 固载葡萄糖氧化酶的壳聚糖/二氧化硅杂化膜的制备及表征 |
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| 引用本文： | 谭学才,王健伟,徐健君,黄婷婷,邹小勇,蔡沛祥.固载葡萄糖氧化酶的壳聚糖/二氧化硅杂化膜的制备及表征[J].中山大学学报(自然科学版),2004,43(4):50-53. |
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| 作者姓名： | 谭学才 王健伟 徐健君 黄婷婷 邹小勇 蔡沛祥 |
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| 作者单位： | 中山大学化学与化学工程学院,广东,广州,510275 |
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| 基金项目： | 广东省自然科学基金，广西自然科学基金 |
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| 摘 要： | 利用生物相容性良好的天然高分子聚合物壳聚糖(CS)与甲基三甲氧基硅烷(MTOS)通过原位溶胶-凝胶法制备壳聚糖/二氧化硅有机无机杂化复合膜,用此膜对葡萄糖氧化酶进行固定,用红外光谱法、扫描电镜法对膜进行了表征.以电沉积普鲁士蓝的玻碳电极和固定化酶膜构建葡萄糖生物传感器,用循环伏安法和记时电流法对固定化酶的效果进行了评价,结果表明,用于研制固定化酶生物传感器时,杂化膜比单纯的壳聚糖膜对底物的响应时间快并能很好地保持酶的活性.
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| 关 键 词： | 壳聚糖/二氧化硅杂化膜 制备 表征 葡萄糖氧化酶电极 固定化 |
| 文章编号： | 0529-6579(2004)04-0050-04 |
| 修稿时间： | 2004年4月8日 |
Preparation and Characterization of Chitosan/Silica Hybrid Film and Its Application in Immobilization of Glucose Oxidase |
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| TAN Xue-cai,WANG Jian-wei,XU Jian-jun,HUANG Ting-ting,ZOU Xiao-yong,CAI Pei-xiang.Preparation and Characterization of Chitosan/Silica Hybrid Film and Its Application in Immobilization of Glucose Oxidase[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2004,43(4):50-53. |
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| Authors: | TAN Xue-cai WANG Jian-wei XU Jian-jun HUANG Ting-ting ZOU Xiao-yong CAI Pei-xiang |
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| Abstract: | A new type of organic-inorganic hybrid film based on sol-gel chitosan /silica was prepared with chitosan(CS) and methytrimethoxysilane (MTOS). The hybrid film was used to immobilize glucose oxidase(GOD) on the surface of Prussian Blue(PB)-modified glass carbon electrode to construct glucose biosensor. The film was studied by fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). Effect of GOD immobilized in film was investigated by cyclic voltammetry and chronoamperometry. The results show that the enzyme electrode fabricated by hybrid film has a fast response time than the enzyme immobilized in pure chitosan film. |
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| Keywords: | chitosan/silica hybrid composite film preparation characterization glucose oxidase electrode immobilization |
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