| 高粱黄酮的提取及其测定方法的建立 |
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| 引用本文： | 袁蕊,敖宗华,丁海龙,徐勇,付勋,沈才洪,黄治国.高粱黄酮的提取及其测定方法的建立[J].北京工商大学学报(自然科学版),2011,29(4):42-45. |
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| 作者姓名： | 袁蕊 敖宗华 丁海龙 徐勇 付勋 沈才洪 黄治国 |
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| 作者单位： | 1. 四川理工学院生物工程学院,四川自贡,643000
2. 泸州老窖股份有限公司,四川泸州,646000 |
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| 基金项目： | 泸州老窖科研奖学金项目“有机原粮收储工艺研究” |
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| 摘 要： | 选用乙醇作为提取溶剂,对高粱中总黄酮进行提取,研究乙醇浓度、浸提温度、浸提时间、料液比对高粱黄酮提取率的影响.并通过单因素及正交试验,确定了高粱黄酮的最佳提取工艺:乙醇浓度为65%,80℃下浸提2 h,料液比为6∶100.此工艺下提取的高粱黄酮含量为0.54%.
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| 关 键 词： | 高粱 黄酮 测定 |
Extraction and Quantitative Determination of Flavonoids in Sorghum |
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| YUAN Rui,AO Zong-hua,DING Hai-long,XU Yong,FU Xun,SHEN Cai-hong,HUANG Zhi-guo.Extraction and Quantitative Determination of Flavonoids in Sorghum[J].Journal of Beijing Technology and Business University:Natural Science Edition,2011,29(4):42-45. |
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| Authors: | YUAN Rui AO Zong-hua DING Hai-long XU Yong FU Xun SHEN Cai-hong HUANG Zhi-guo |
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| Institution: | YUAN Rui1,AO Zong-hua2,DING Hai-long2,XU Yong2,FU Xun1,SHEN Cai-hong2,HUANG Zhi-guo1(1.College of Bioengineering,Sichuan University of Science and Engineering,Zigong 643000,China,2.Luzhou Laojiao Co.Ltd.,Luzhou 646000,China) |
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| Abstract: | Extraction of flavonoids in sorghum using ethanol was studied. The effects of ethanol concen- tration, temperature, extraction time, and ratio of material to solvent were studied. The optimum conditions of extraction were obtained as follows： 65% ethanol, solid/liquid ratio of 6： 100, extraction time of 120 rain under 80^oC. Under the optimal conditions, the content of total flavonoids was O. 54% |
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| Keywords: | sorghum flavonoids determination |
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