犬细小病毒新抗原变异株VP2基因的克隆与序列分析

目的鉴定犬细小病毒(Canine parvovirus,CPV)新抗原变异株的病毒型,为研究CPV变异及制备CPV特异性诊断和治疗试剂奠定基础。方法从病毒细胞培养物中提取基因组DNA,设计合成针对VP2基因的1对特异引物,PCR扩增出VP2基因片段,克隆后测序,应用DNAstar进行序列分析。结果得到CPV-VP-2基因序列15份,其中CPV-2a 10份,CPV-2b 5份;FPV-VP-2基因1份。结论各序列与已发表的标准序列比较,同源性在98%以上,未形成明显分支。

Clone and Sequence Analysis of New Antibody-Variant of Canine Parvovirus

Objective To identify the antigenic and evolution to study the mutation of CPV and lay the foundation for the identification and treatment of this disease. Methods A pair of primers were designed and synthesized based on VP2 gene of Canine parvovirus （CPV）. The genomic DNA samples of CPV strain isolated from culture solution ware extracted and used as templates for PCR to clone the VP2 gene. The sequences of the DNA fragment were aligned and analyzed by DNAstar. Results 15 were CPV-VP-2, including 10 of CPV-2a and 5 of CPV-2b; 1 was FPV-VP-2. Conclusions Compared with standard sequence, homogeneities were above 98 %, they had not formed obvious branch.