| 组合浸矿细菌菌群的氧化特性及其群落结构分析 |
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| 引用本文： | 李乾,蒋潇宇,王星星,王清良,胡鄂明,史文革.组合浸矿细菌菌群的氧化特性及其群落结构分析[J].南华大学学报(自然科学版),2017,31(3):25-28. |
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| 作者姓名： | 李乾 蒋潇宇 王星星 王清良 胡鄂明 史文革 |
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| 作者单位： | 南华大学 铀矿冶生物技术国防重点学科实验室,湖南 衡阳421001;南华大学 核资源工程学院,湖南 衡阳421001;南华大学 矿业工程博士后流动站,湖南 衡阳421001,南华大学 铀矿冶生物技术国防重点学科实验室,湖南 衡阳421001;南华大学 核资源工程学院,湖南 衡阳421001,南华大学 铀矿冶生物技术国防重点学科实验室,湖南 衡阳421001;南华大学 核资源工程学院,湖南 衡阳421001,南华大学 铀矿冶生物技术国防重点学科实验室,湖南 衡阳421001;南华大学 核资源工程学院,湖南 衡阳421001,南华大学 铀矿冶生物技术国防重点学科实验室,湖南 衡阳421001;南华大学 核资源工程学院,湖南 衡阳421001,南华大学 铀矿冶生物技术国防重点学科实验室,湖南 衡阳421001;南华大学 核资源工程学院,湖南 衡阳421001 |
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| 基金项目： | 中国博士后科学基金(2014M562116)；湖南省自然科学基金(14JJ6024)；南华大学核资源与环境大学生创新训练中心创新实验项目 |
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| 摘 要： | 为了探究组合浸矿细菌菌群的氧化特性及其群落结构,构建4株浸矿细菌(Acidithiobacillus ferrooxidans ATCC 23270,Leptospirillum ferriphilum YSK,Acidithiobacills thiooxidans A01,Acidithiobacills caldus S1)的组合浸矿菌群.实验结果表明:此组合浸矿细菌菌群中的菌体具有较高的生物活性,表现出良好的的铁、硫氧化能力.并采用实时荧光定量PCR技术对菌群的群落结构进行了分析.结果表明:此体系中A.ferrooxidans ATCC 23270、A.thiooxidans A01是优势种群;L.ferriphilum YSK和A.caldus S1是劣势种群.
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| 关 键 词： | 浸矿细菌菌群 铁/硫氧化 群落结构 |
| 收稿时间： | 2017/5/5 0:00:00 |
Oxidation Characteristics and Community Structure ofa Mixed Bioleaching Bacterial Consortium |
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| LI Qian,JIANG Xiao-yu,WANG Xing-xing,WANG Qing-liang,HU E-ming and SHI Wen-ge.Oxidation Characteristics and Community Structure ofa Mixed Bioleaching Bacterial Consortium[J].Journal of Nanhua University:Science and Technology,2017,31(3):25-28. |
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| Authors: | LI Qian JIANG Xiao-yu WANG Xing-xing WANG Qing-liang HU E-ming and SHI Wen-ge |
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| Institution: | Key Discipline Laboratory for National Defence of Biotechnology in Uranium Mining and Hydrometallurgy,University of South China,Hengyang,Hunan 421001,China;School of Nuclear Resources Engineering,University of South China,Hengyang,Hunan 421001,China;Postdoctoral Research Station of Mineral Engineering,University of South China,Hengyang,Hunan 421001,China,Key Discipline Laboratory for National Defence of Biotechnology in Uranium Mining and Hydrometallurgy,University of South China,Hengyang,Hunan 421001,China;School of Nuclear Resources Engineering,University of South China,Hengyang,Hunan 421001,China,Key Discipline Laboratory for National Defence of Biotechnology in Uranium Mining and Hydrometallurgy,University of South China,Hengyang,Hunan 421001,China;School of Nuclear Resources Engineering,University of South China,Hengyang,Hunan 421001,China,Key Discipline Laboratory for National Defence of Biotechnology in Uranium Mining and Hydrometallurgy,University of South China,Hengyang,Hunan 421001,China;School of Nuclear Resources Engineering,University of South China,Hengyang,Hunan 421001,China,Key Discipline Laboratory for National Defence of Biotechnology in Uranium Mining and Hydrometallurgy,University of South China,Hengyang,Hunan 421001,China;School of Nuclear Resources Engineering,University of South China,Hengyang,Hunan 421001,China and Key Discipline Laboratory for National Defence of Biotechnology in Uranium Mining and Hydrometallurgy,University of South China,Hengyang,Hunan 421001,China;School of Nuclear Resources Engineering,University of South China,Hengyang,Hunan 421001,China |
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| Abstract: | In order to explore the oxidation characteristics and community structure of mixed bioleaching bacterial consortia,a bacterial consortium including four typical bioleaching strains (Acidithiobacillus ferrooxidans ATCC 23270,Leptospirillum ferriphilum YSK,Acidithiobacills thiooxidans A01,Acidithiobacills caldus S1) was constructed.The results show that strains in bioleaching bacterial consortium exert high biological activity and great oxidation ability to iron and sulfur.Microbial community structure was analyzed by real-time fluorescent quantitative PCR technology.The results show that A.ferrooxidans ATCC 23270 and A.thiooxidans A01 are the dominant species,while the minor is L.ferriphilum YSK and A.caldus S1. |
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| Keywords: | bioleaching bacterial consortium iron/sulfur oxidation capability communitystructure |
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