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混合菌固定化及其对土壤中芘和苯并芘的降解
引用本文:苏丹,李培军,王鑫,V.A.Verkhozina.混合菌固定化及其对土壤中芘和苯并芘的降解[J].辽宁工程技术大学学报(自然科学版),2007,26(3):461-463.
作者姓名:苏丹  李培军  王鑫  V.A.Verkhozina
作者单位:1. 中国科学院,沈阳应用生态研究所,辽宁,沈阳,110016;中科院研究生院,北京,100049
2. 中国科学院,沈阳应用生态研究所,辽宁,沈阳,110016
3. 沈阳大学沈阳环境工程重点实验室,辽宁,沈阳,110044
4. 俄罗斯科学院,西伯利亚分院,664033,伊尔库茨克市,俄罗斯
基金项目:国家重点基础研究发展计划973项目(2004CB418506),国家高科技研究发展计划“863”(2004AA649060),国家重点基金资助项目(20337010)
摘    要:从石油污染土壤中筛选出1株细菌(Bacillus sp.)和1株真菌(Mucor sp.),以12种不同材料为载体对混合菌进行固定化,研究了固定化混合菌的降解特性.结果表明,采用吸附法能有效实现混合菌在改性后蛭石上的固定化.制得的固定化混合菌,传质性能好,对芘(Pyr)和苯并芘(BaP)的降解率42 d分别可达94.96%和74.96%,明显高于游离菌对Pyr和BaP的降解率60.49%和50.09%.采用扫描电子显微镜(SEM)观察了固定化混合菌微观结构,同时探讨了固定化混合菌的传质过程

关 键 词:固定化  降解    苯并芘  土壤污染  蛭石
文章编号:1008-0562(2007)03-0461-03
修稿时间:2006-07-02

Immobilization of combined bacteria and its degradation of pyrene and benzo[a]pyrene in contaminated soil
SU Dan,LI Pei-jun,Wang Xin,V.A.Verkhozina.Immobilization of combined bacteria and its degradation of pyrene and benzo[a]pyrene in contaminated soil[J].Journal of Liaoning Technical University (Natural Science Edition),2007,26(3):461-463.
Authors:SU Dan  LI Pei-jun  Wang Xin  VAVerkhozina
Institution:1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 2. Gradate University of Chinese Academy of Sciences, Beijing 100049 China; 3. Shenyang Key Laboratory of Envrionmental Engineering, Shenyang University, Shenyang 110044,China; 4. Institute of Geochemistry, by A.P.Vinogradova Siberian Branch of Russian Academy of Sciences 664033,Irkutsk,Russian
Abstract:A strain autochthonous fungus and a strain bacterium identified as Bacillus sp.and Mucor sp.were isolated from a contaminated soil and their co-immobilization was studied.The co-immobilization carrier and the method was investigated,which could after screening degrade pyrene(Pyr) and benzoa]pyrene(BaP) in the contaminated soil efficiently.The results show that the optimumal immobilized carrier was modified vermiculite,and better mass transfer and could degrade 94.96% and 74.96% of Pyr and BaP respectively in 42 days,which was remarkably higher than the degradation rate of that by the free strains.The microstructure of immobilized mixture microorganism was observed by scanning electron microscope(SEM).Also,the process of the transmission in soil was discussed.
Keywords:immobilization  biodegradation  pyrene  benzo[a]pyrene  contaminated soil  vermiculite
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