| 荧光素二聚体荧光探针同步荧光法测定阿米卡星 |
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| 引用本文： | 陈培珍,;林旭聪,;谢增鸿.荧光素二聚体荧光探针同步荧光法测定阿米卡星[J].漳州师院学报,2009(3):95-98. |
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| 作者姓名： | 陈培珍 ;林旭聪 ;谢增鸿 |
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| 作者单位： | [1]福建武夷学院化学与环境工程系,福建武夷山354300; [2]福州大学化学化工学院,福建福州350002 |
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| 基金项目： | 国家自然基金（20575012）;福建省科技项目（2007Y0062）;南平市科技项目（N2005202） |
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| 摘 要： | 基于阿米卡星（AMK）能增强荧光素二聚体的荧光信号，建立了以荧光素二聚体荧光探针同步荧光光谱技术测定AMK的新方法．结果表明，在△λ=30nm，最优条件下，测定AMK的线性范围为0．5～20．0μg／mL，检出限（LOD）为0．51μg／ml．用于实际样品分析，测定回收率为96．9％-104．5％，相对标准偏差（RSD）为3．7％～5．3％．
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| 关 键 词： | 阿米卡星 荧光素二聚体 同步荧光光谱 |
Determination of Amikacin by Synchronous Fluorescence Technique with Fluorescein Dimer as a Probe |
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| Institution: | CHEN Pei-zhen , LIN Xu-cong, XIE Zeng-hong. (1.Chemistry and Environment Engineering Department, Wuyi University, Wuyishan, Fujian 363000, China; 2.Chemistry and Engineering College, Fnzhou University, Fuzhou, Fujian 363000, China) |
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| Abstract: | Base on the signal of fluorescein Dimer can be enhanced by Amikacin, A new method that taking Fluorescein Dimer as a fluorescence probe to determine Amikacin was built .The result showed that under the optimal condition of △k=30pm, good calibration curves of the Amikacin were obtained in the range of 0.5-20.0μg/mL with detection limits （LOD） of 0.51μg/ml. Applied to the commercial samples, this method was adaptable. And the recoveries ranged from 96.9% to 104.5% and RSD from 3.7% to 5.3%. |
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| Keywords: | amikacin fluorescein dimmer synchronous fluorescence spectrum |
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