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| 饲用复合酶生产菌株的诱变选育 | |
| 引用本文： | 王世英,雷白时,袁洪水,李术娜,朱宝成.饲用复合酶生产菌株的诱变选育[J].河北大学学报(自然科学版),2006,26(1):81-85. |
| 作者姓名： | 王世英 雷白时 袁洪水 李术娜 朱宝成 |
| 作者单位： | 1. 河北农业大学,生命科学学院,河北,保定,071001 2. 河北大学,生命科学学院,河北,保定,071002 3. 河北农业大学,生命科学学院,河北,保定,071001;河北大学,生命科学学院,河北,保定,071002 |
| 基金项目： | 河北省科学技术研究与发展计划 |
| 摘 要： | 通过UV诱变复合酶产生菌黑曲霉筛选得到一突变株JW001,该菌株可同时发酵产生高活力的多酶系,其中纤维素酶和酸性蛋白酶活力分别为18 379 u/g和6 472 u/g,比出发菌株分别提高122%和92%.优化确定了曲盘固体发酵工艺,并进行了复合酶动物应用试验,试验表明黑曲霉JW001菌株发酵生产的饲用复合酶制剂对不同畜禽均有很好的饲喂效果. |
| 关 键 词： | 黑曲霉 突变菌株 饲用复合酶 固体发酵 |
| 文章编号： | 1000-1565(2006)01-0081-05 |
| 修稿时间： | 2005年8月30日 |
Mutagenesis Breeding of the Feed Enzyme-complex Producing Strain |
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| WANG Shi-ying,LEI Bai-shi,YUAN Hong-shui,LI Shu-na,ZHU Bao-cheng.Mutagenesis Breeding of the Feed Enzyme-complex Producing Strain[J].Journal of Hebei University (Natural Science Edition),2006,26(1):81-85. | |
| Authors: | WANG Shi-ying LEI Bai-shi YUAN Hong-shui LI Shu-na ZHU Bao-cheng |
| Abstract: | One feed complex-enzyme producing mutant strain of Aspergillus niger JW001 was obtained by UV mutagenesis,the strain can produce high activity of multi-enzyme systems.The cellulase enzyme activity was 18 379 u/g,122% higher than original strain and the acidic proteinase enzyme activity was 6 472 u/g,92%higher than original strain.The solid plate fermentation process of this strain was optimized and animal feeding experiments were carried out,the results showed that the feed enzyme-complex produced by strain JW001 could bring series beneficial effects on different animals. |
| Keywords: | Aspergillus niger mutant strain feed enzyme-complex solid fermentation |
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