| 外界因子对粘毛黄芩毛状根生长和黄酮类化合物合成的影响 |
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| 引用本文： | 王淑芳,张仁波,窦全丽.外界因子对粘毛黄芩毛状根生长和黄酮类化合物合成的影响[J].河南师范大学学报(自然科学版),2012,40(4):124-126. |
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| 作者姓名： | 王淑芳 张仁波 窦全丽 |
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| 作者单位： | 1. 嘉兴学院生物与化学工程学院,浙江嘉兴,314001
2. 遵义师范学院生物系,贵州遵义,563002 |
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| 摘 要： | 目的:研究不同外界因子对粘毛黄芩毛状根生长和黄酮类化合物合成的影响.方法:研究不同基本培养基、pH值、碳源及激素对粘毛黄芩毛状根生长和黄酮类化合物合成的影响.结果:30g.L-1蔗糖、pH 5.8的1/2MS培养基最适合毛状根生长,但黄酮类化合物合成最高时pH值为6.4.外源激素NAA、GA对毛状根的生长有促进作用,GA效果最为明显.结论:初步优化了粘毛黄芩毛状根的培养条件,为实现粘毛黄芩黄酮类化合物大规模生产奠定了基础.
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| 关 键 词： | 粘毛黄芩 毛状根 黄酮类化合物 |
Effects of Different Factors on Hairy Root Growth and Flavonoids Biosynthesis in the Cultures of Scutellaria Viscidula BgeHairy Root |
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| WANG Shu-fang
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ZHANG Ren-bo
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DOU Quan-li.Effects of Different Factors on Hairy Root Growth and Flavonoids Biosynthesis in the Cultures of Scutellaria Viscidula BgeHairy Root[J].Journal of Henan Normal University(Natural Science),2012,40(4):124-126. |
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| Authors: | WANG Shu-fang ZHANG Ren-bo DOU Quan-li |
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| Institution: | 1.College of Biological and Chemicel Engineering,School of Jiaxing University Jiaxing 314001,China; 2.Biology Department,Zunyi Normal College,Zunyi 563002,China) |
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| Abstract: | Objective:To study the effects of different physical and chemical factors on hairy root growth and flavonoids production in suspension culture of Scutellaria viscidula Bge hairy root.Methods : To study hairy root growth and flavonoids production in factors of the media used,pH values,sources of carbon and exogenous phytohormones.Results: The best conditions of hairy root culture were 1/ 2MS medium with sucrose(3%) as carbon material and pH 5.8,but the optimum pH for flavonoids production was 6.4;NAA and GA could improve the growth speed of the hairy root,while the most obvious effect is GA.Conclusion:Eminent Cultured-conditions of hairy root give a new pathway to resolve the shortage of baicalin of Scutellaria viscidula Bunge. |
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| Keywords: | Scutellaria viscidula Bge hairy root flavonoids |
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