藻菌共生体系的建立及其对造纸废水的处理效果

对棕鞭藻(Ochromonas sp.)培养体系中的共栖细菌进行分离纯化,拟通过藻菌共生体系的建立提高微藻生物量积累能力,并探究藻菌共生体系对造纸废水的处理效果.经16S rDNA基因测序比对,分离获得的5株微藻共栖细菌分别为Por-phyrobacter sp.、Hyphomonas sp.、Aquimonas sp.、Agrobacterium sp.和Hydrogenophaga sp..通过向微藻培养体系添加不同的共栖细菌以验证其对微藻生长效率的影响效果,结果表明,5株微藻共栖细菌中,Aquimonas sp.(水单胞菌)可显著促进棕鞭藻的生长,当藻菌比为1:3时,水单胞菌对微藻的促生效果最佳,共培养10 d后,体系中微藻干重达到最大值0.78 g/L.利用藻菌共生体系处理造纸废水8 d,废水中化学需氧量(chemical oxygen demand,COD)、总氮量(total nitrogen,TN)、总磷量(total phos-phorus,TP)、色度分别从154.13 mg/L、22.42 mg/L、4.90 mg/L、275降至37.5 mg/L、14.53 mg/L、0.48 mg/L、18,去除率分别为75.67％、35.19％、90.2％和93.45％;同时微藻干重可达1.073 g/L.藻菌共生体系对造纸废水深度处理的效果与芬顿法相当,可达到《制浆造纸工业水污染排放标准》(GB 3544—2008),同时可显著降低处理成本,具有良好的应用前景.

Construction of Microalgae-bacteria Symbiosis System and the Treatment effect on Papermaking Wastewater

Symbiotic bacteria were isolated and purified from the Ochromonas sp. culture system. It was proposed to improve the capacity of microalgae biomass accumulation through the establishment of algae-bacteria symbiosis system. And the treatment effect of the algae-bacteria symbiosis system on papermaking wastewater was explored. Five strains of bacteria were identified as Porphyrobacter sp. (A), Hygromonas sp. (B), Aquimonas sp. (C), Agrobacterium sp. (D) and Hydrophaga sp. (E) by 16S rDNA sequencing technology. In order to verify the effect on the growth efficiency of microalgae, different bacteria were added to the culture system of microalgae. The results showed that Aquimonas sp. could significantly promote the growth of Ochromonas sp. among the five bacterial strains. And the best growth-promoting effect was showed when the algae/bacteria ratio was 1:3. The dry weight of Ochromonas sp. increased to 0.78 g/L on the 10th day of cultivation. The wastewater was treated by symbiotic system in 8 days, the concentration of COD, TN , TP and chroma could be reduced from 153.45 mg/L, 31.58 mg/L , 4.83 mg/L and 275 to 37.5 mg/L, 20.53 mg/L , 0.48 mg/L and 18, with the removal rates of 75.67%, 35.19% , 90.2% and 93.45% respectively. At the same time, the content of dry weight in microalgae was 1.073 g/L. The effect of microalgae-bacteria symbiosis system on the advanced treatment of papermaking wastewater is similar to Fenton Method and with lower cost, which can meet the Pulp and Paper Industry Water Pollution Discharge Standard (GB3544-2008) and have wide perspective on the treatment of wastewater.