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3月龄大鼠不同大动脉光镜和CLSM观察及定量分析
引用本文:于鹏,李静,王琳,段相林.3月龄大鼠不同大动脉光镜和CLSM观察及定量分析[J].河北师范大学学报(自然科学版),2002,26(3):297-300.
作者姓名:于鹏  李静  王琳  段相林
作者单位:1. 河北师范大学生命科学学院,河北石家庄,050016
2. 石家庄市第11中学,河北石家庄,050000
摘    要:采取常规石蜡切片和HE染色法制成切片标本,在光镜和激光共聚扫描显微镜(CLSM)下测定升主动脉、胸主动脉、腹主动脉和股动脉的管壁中膜厚度、管腔面积、管壁中膜面积、弹性膜层数和弹性膜荧光强度等,结果表明了由升主动脉至股动脉呈现血管壁中膜变薄、管腔缩小、弹性膜荧光强度降低等变化规律。

关 键 词:3月龄大鼠  大动脉  CLSM观察  定量分析  弹性膜  弹性动脉  光镜观察  膜厚度  管腔面积  显微结构
文章编号:1000-5854(2002)03-0297-04
修稿时间:2001年9月27日

LM and CLSM Observation and Quantitative Analysis of Different Large Arteries in Rats of Three Months Old
YU Peng ,LI Jing ,WANG Lin ,DUAN Xiang-lin.LM and CLSM Observation and Quantitative Analysis of Different Large Arteries in Rats of Three Months Old[J].Journal of Hebei Normal University,2002,26(3):297-300.
Authors:YU Peng  LI Jing  WANG Lin  DUAN Xiang-lin
Institution:YU Peng 1,LI Jing 1,WANG Lin 2,DUAN Xiang-lin 1
Abstract:In this experiment,conventional hematoxylin-eosin staining was used to make sections,then with light microscope and confocal laser scanning microscope the changes of different elastic arteries were investigated and measured.The results are as follows:from ascending aoerta to femoral artery,both the area and the thickness of tunica media of vascular wall were obviously decreased,so was the area of vascular cavity;while the ratio of vascular wall area to vascular cativity area was obviously increased;fluorescence intensity of elastic membranes showed a tendency to decrease.In a word,great changes take place in different elastic arteries.
Keywords:elastic membrane  confocal laser scanning microscope  elastic artery  SD rat
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