| Myotoxic activity of a Gln-49 phospholipase A_2 from Agkistrodon blomhoffii ussurensis snake venom |
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| 作者姓名： | BAO Yongming BU Pengcheng YANG Jun YANG Wei JIA Peilin and AN Lijia |
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| 作者单位： | Department of Bioscience and Biotechnology，Dalian University of Technology，Dalian 116024，China; Institute of Biophysics，Chinese Academy of Sciences，Beijing 100101，China; Graduate School of Chinese Academy of Sciences，Beijing 100039，China |
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| 基金项目： | Dalian Science and Technology Bureau |
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| 摘 要： | A novel myotoxic protein phospholipase A2(PLA2), denoted as Gln49-PLA2, has been isolated from snake venom of Agkistrodon blomhoffii ussurensis, which has weak lethal effect and apparent anticoagulant activity, but lacks the PLA2 and hemorrha-genic activity. Gln49-PLA2 obviously increases of plasma creatine-kinase (CK) upon intramuscular injection in mice, suggesting that it may induce a dose-dependent myonecrosis. Histological studies also reveal morphological changes in mouse skeletal muscles, including extensive myonecrosis, hemorrhage and neutrophil infiltration in the treated animals. The myotoxic ability induced by Gln49-PLA2 can be partially inhibited by heparin.
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Myotoxic activity of a Gln-49 phospholipase A2 from Agkistrodon blomhoffii ussurensis snake venom |
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| BAO Yongming,BU Pengcheng,YANG Jun,YANG Wei,JIA Peilin and AN Lijia.Myotoxic activity of a Gln-49 phospholipase A_2 from Agkistrodon blomhoffii ussurensis snake venom[J].Progress in Natural Science,2006,16(2). |
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| Authors: | BAO Yongming BU Pengcheng ANG Jun YANG Wei JIA Peilin AN Lijia |
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| Abstract: | A novel myotoxic protein phospholipase A2(PLA2), denoted as Gln49-PLA2, has been isolated from snake venom of Agkistrodon blomhoffii ussurensis, which has weak lethal effect and apparent anticoagulant activity, but lacks the PLA2 and hemorrha-genic activity. Gln49-PLA2 obviously increases of plasma creatine-kinase (CK) upon intramuscular injection in mice, suggesting that it may induce a dose-dependent myonecrosis. Histological studies also reveal morphological changes in mouse skeletal muscles, including extensive myonecrosis, hemorrhage and neutrophil infiltration in the treated animals. The myotoxic ability induced by Gln49-PLA2 can be partially inhibited by heparin. |
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| Keywords: | snake venom phospholipase A2 Agkistrodon blomhoffii ussurensis myotoxic activity |
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