| 一种快速、经济、有效纯化人工合成寡核苷酸片段的方法 |
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| 引用本文： | 陈劲春,李宏帆.一种快速、经济、有效纯化人工合成寡核苷酸片段的方法[J].首都师范大学学报(自然科学版),1997,18(1):82-84. |
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| 作者姓名： | 陈劲春 李宏帆 |
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| 作者单位： | 首都师范大学生物系 |
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| 基金项目： | 北京市自然科学基金委资助项目 |
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| 摘 要： | 采用DEAE-52纤维紊小柱，纯化了7个人工合成的寡核苷酸引物．纯化效果栗用岛津(uy-265)分光光度计扫描，8mol／L尿素-12%聚丙烯酰胺凝胶电泳及自提的模板DNA经PCR检测．该法分传统的HPLC法及PAGE法相比，具有快速、经济、有效的优越性。
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| 关 键 词： | 寡核苷酸片段 纯化效果 离子交换层析柱 引物 分光光度计 高效液相法 聚丙烯酰胺凝胶电泳法 |
A Rapid,Economic and Efficient Method for Purafication of Oligonucleotide Primers |
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| Chan Jinchun Li Hongfan.A Rapid,Economic and Efficient Method for Purafication of Oligonucleotide Primers[J].Journal of Capital Normal University(Natural Science Edition),1997,18(1):82-84. |
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| Authors: | Chan Jinchun Li Hongfan |
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| Abstract: | Seven synthesised oligonucleotide primers were purified with a small column of DEAE-52 cellulose at our laboratory. The results were scanned with UV-265 spectrophotometer. 12% of denatured get of polyacrylamide were also Cheeked by PCR. Comparison of the popular methods of HPLC and ACGE,this method was more rapid,economic and effident. |
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| Keywords: | DEAE-52 cellulose purification primer |
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