中华眼镜蛇毒神经生长因子的研究

采用ＣＭ－ＳｅｐｈａｒｏｓｅＣＬ－６Ｂ和ＳｅｐｈｏｓｉｌＣ８以及ＨＰＬＣ层析方法，自广西产中华眼镜蛇毒中分离纯化神经生长因子（ＮＧＦ），此ＮＧＦ经８ｄ鸡胚背根神经节体外培养证明具有促进神经纤维生长的活性。经ＨＰＬＣ及聚丙烯酰胺凝胶电泳鉴定为单一组成，经ＳＤＳＰＡＧＥ及ＨＰＬＣ测定分子量分别为２４．１ＫＤ和２４．９ＫＤ，等电聚焦电泳测得ｐＩ为８．２，氨基酸组分分析表明ＮＧＦ含酸性氨基酸较少，通过１２５｜标记ＮＧＦ测定出ＮＧＦ在生物体内主要分布于肾、肺、及周围神经

The Study of Nerve Growth Factor in Chinese Cobra

Nerve growth factor (NGF) was purified from the venom of Chinese Cobra (Naja naja atra) by the CM Sepharose CL-6B column chromatography and sephasil C8 reversed-phase chromatography,and then by the HPLC. The purified NGF was shown to be homogeneous in discontinuous PAGE,in SDS-PAGE and in High-pressure Liquid Chromatography (HPLC). In vitro, the greatest outgrowth of the dorsal root ganglias of chicken embryos invoked by NGF at a concentration of 100 ng/mL. The molecular weight was estimated to be about 24.9 KD by HPLC, and to be 24.1 KD by SDS-PAGE. The isoelectric point was about 8.2. Amino acid analysis showed that NGF contained less residues of acidic amino acid.In vivo, the distribution of ¹²⁵|-NGF was found to be concentrated in kidney, peripheral nerve, liver and lung.