|
|||||
|
|
| 树鼩对人乙型肝炎病毒易感性的验证 | |
| 引用本文： | 苏建家,李瑗,王树声,班克臣,覃柳亮,欧超,张华.树鼩对人乙型肝炎病毒易感性的验证[J].广西科学,2000,7(4):298-301. |
| 作者姓名： | 苏建家 李瑗 王树声 班克臣 覃柳亮 欧超 张华 |
| 作者单位： | 1. 广西壮族自治区防疫站，广西南宁，530021。 2. 中国药品生物制品检定所，北京 3. 广西肿瘤防治研究所南宁市滨湖路6号 530021 |
| 基金项目： | 国家科技攻关项目，96-906-03-10， |
| 摘 要： | 为验证树Ｑｕ对人乙型肝炎病毒（ＨＢＶ）的易感性，用含ＨＢＶ的人血清接种给１０只成年树Ｑｕ（雌雄各半）。然后每周抽血１次，每只动物共抽血１１次。用不同公司生产的ＥＬＩＳＡ试剂检测接种后的动物血清感染指标。实验观察至１３周，结果分别有９只和８只动物出现ＨＢｓＡｇ阳性，持续最短１周，最长７周。用ＰＣＲ检测，结果有１只动物连续４周在血清中检测出ＨＢＶ ＤＮＡ。表明树Ｑｕ能感染人ＨＢＶ，但实验有待改进以延长感染持续时间。 |
| 关 键 词： | 树Qu 乙型肝炎病毒 感染 易感性 ALT检测 HBV |
| 收稿时间： | 2000/7/4 0:00:00 |
| 修稿时间： | 2000年7月4日 |
Susceptibility Inspecting for the Human Hepatitis B Virus Infection in Tree Shrews |
|
| Su Jianji,Li Yuan,Wang Shusheng,Ban Kechen,Qin Liuliang,Ou Chao and Zhang Huayuan.Susceptibility Inspecting for the Human Hepatitis B Virus Infection in Tree Shrews[J].Guangxi Sciences,2000,7(4):298-301. | |
| Authors: | Su Jianji Li Yuan Wang Shusheng Ban Kechen Qin Liuliang Ou Chao and Zhang Huayuan |
| Institution: | Guangxi Cancer Institute, 6 Binhulu, Nanning, Guangxi, 530021, China,Guangxi Cancer Institute, 6 Binhulu, Nanning, Guangxi, 530021, China,Guangxi Cancer Institute, 6 Binhulu, Nanning, Guangxi, 530021, China,Guangxi Cancer Institute, 6 Binhulu, Nanning, Guangxi, 530021, China,Guangxi Cancer Institute, 6 Binhulu, Nanning, Guangxi, 530021, China,Guangxi Cancer Institute, 6 Binhulu, Nanning, Guangxi, 530021, China and Guangxi Cancer Institute, 6 Binhulu, Nanning, Guangxi, 530021, China |
| Abstract: | To examine the tree shrew''s susceptibility for human hepatitis B virus,ten adult tree shrews (5 males,5 females) were inoculated by human sera with HBV. After that, weekly blood sampling was carried out one time each animal lasted for 11 weeks. HBsAg was detected with ELISA kits purchased from two different companies. Results showed that 9 and 7 animals in this experiment were positive for HBsAg respectively. One animal was found HBV DNA in sera by PCR, which lasted for 4 weeks. The data indicates that tree shrew could be infected with human HBV, but the methods of inoculation or conditions need to be improved to achieve a chronic HBV infection with more persistence of viral replication. |
| Keywords: | tree shrew hepatitis B virus infection |
| 本文献已被 维普 万方数据 等数据库收录！ | |
| 点击此处可从《广西科学》浏览原始摘要信息 | |
| 点击此处可从《广西科学》下载免费的PDF全文 | |