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| 乙二醇玻璃化冷冻液二步法对长爪沙鼠 2-细胞胚胎的冷冻保存 | |
| 引用本文： | 郭红刚,卢领群,周生来,戴方伟,李巍,李长龙,陈振文,萨晓婴.乙二醇玻璃化冷冻液二步法对长爪沙鼠 2-细胞胚胎的冷冻保存[J].实验动物科学,2014,31(4):28-31. |
| 作者姓名： | 郭红刚 卢领群 周生来 戴方伟 李巍 李长龙 陈振文 萨晓婴 |
| 作者单位： | 1. 浙江省医学科学院,杭州,310013 2. 中国医科大学实验动物部,沈阳,110001 3. 首都医科大学基础医学院,北京,100069 |
| 基金项目： | 国家科技支撑计划重点项目，浙江省级公益性技术应用研究计划，浙江省卫生厅项目 |
| 摘 要： | 摘要: 目的 评价用乙二醇玻璃化冷冻液二步法对长爪沙鼠 2-细胞胚胎冷冻保存的效果。方法 通过超排卵处理 后与雄鼠交配获得长爪沙鼠 2-细胞胚胎，胚胎在冷冻液 EFS20 中平衡 3 min，然后移入冷冻液 EFS40 中平衡 1 min， 之后将细胞冻存管直接浸入液氮中保存。采用二步法复苏胚胎，复苏后移入改良的胚胎培养液( mKSOM) 中体外 培养。结果 采用该方法冷冻的胚胎复苏后胚胎回收率为 86. 73% ，存活率为 82. 94% ，复苏后存活的胚胎中约 1 / 3 胚胎可以在体外发育到囊胚。结论 乙二醇玻璃化冷冻液适于冷冻保存长爪沙鼠 2-细胞胚胎。 |
| 关 键 词： | 玻璃化冷冻 冷冻保存 2-细胞胚胎 长爪沙鼠 |
Cryopreservation of Mongolian gerbil 2-cell Embryos by Vitrification based on EG with Two Steps |
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| Institution: | GUO Hong-gang , LU Ling-qun , ZHOU Sheng-lai , DAI Fang-wei , LI Wei , LI Chang-long, CHEN Zhen-wen ,SA Xiao-ying ( 1. Zhejiang Academy of Medical Sciences,Hangzhou 310013, China) (2. Laboratory Animal Center, China Medical University, Sheayang 110001, China ) ( 3. Department of Laboratory Animal Science, Capital Medical University, Beijing 100086, China ) |
| Abstract: | Objective Using the thyleneglycol（EG）-based solutions with two steps to freeze the 2-cell embryos of Mongolian gerbil（Meriones unguiculatus） and to appraise the effect of embryo cryopreservation. Method The embryos were exposed serially to EFS20 for 3 minutes and EFS40 for 1 minute. The embryo trealed were immersed into liquid nitrogen. Result The embryo recovery rate was 86.73% and after freezing-thawing it survival rate was 82.94%. And about 30% of the freezing-thawing embryos developed to blastocyst in vitro. Conclusion This results demonstrates that Mongolian gerbil embryos can be safely eryopreserved using vitrification solutions based on EG with two steps. |
| Keywords: | vitrification cryopreservation 2-cell embryo Mongolian gerbil(Meriones unguiculatus) |
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