6个小鼠ES细胞系的建立和鉴定

报道从小鼠129/ter品系中建成6个胚胎干细胞系，经过核型检查，体内、体外分化等鉴定后发现，6个细胞系中有2个细胞系是正常二倍体，其中ES细胞系MESPU 13为XY型，其核骨架蛋白质成分相当于3～4d(天)的鼠胚细胞的核骨架成分，可长期维持二倍体未分化状态，并具有高效的嵌合能力。核型不正常的细胞系所形成的畸胎瘤生长较快，但细胞的分化程度与核型正常的ES细胞有所不同。对影响建系结果的各种因素进行了讨论。

Establishment and Characterization of Six ES Cell Lines from Mouse 129/ter Strain

Six mouse Smbryonic stem cell lines were established from strain129/ter.Their karyotypes as well as their differentiation ability in vivo and in vitrohad been tested.Two of the six cell lines had normal diploid karyotypes,andMESPU 13 was a XY ES cell line.MESPU 13 could keep its normal karyotype andundifferentiated state over long period of culture,and it was highly efficient informing chimeras.The cell skeleton protein content of MESPU 13 was the same as that of the 3~4 days old murin embryonic cells.The teratomas of the karyotypical-ly abnormal ES cell lines grew faster,but their differentiation extent was slightlydifferent from those of the normal ones.The factors which might affect the estab-lishment of ES cell lines had also been discussed.