微波-超声波辅助提取金丝小枣环磷酸腺苷的技术研究

为提高金丝小枣环磷酸腺苷提取率,以金丝小枣为原料,通过Plackett-Burman(PB)实验设计,确定了影响金丝小枣环磷酸腺苷提取率的显著因素为微波时间、料液比、超声时间和超声功率。在此基础上,通过Box-Behnken(BB)实验设计、响应面分析法,最终得出了超声时间是影响提取效果的最显著因子,其次是料液比,微波时间、超声功率在设定条件范围内对提取效果影响不显著。确定了金丝小枣环磷酸腺苷的最佳提取条件为微波功率440 W、微波时间64 s、料液比1:8、超声时间21 min、超声功率300 W、超声温度20℃,在此条件下,金丝小枣环磷酸腺苷提取率为90.83%。本研究成果能够为医药和食品工业提供良好的天然活性物质,为金丝小枣及其他枣品的深加工和工业化应用提供了参考依据。

Microwave-ultrasonic aided extraction for cAMP from Ziziphus jujube

To improve the extraction rate of cAMP from Ziziphusjujube, we identified the significant factors affecting cAMP extraction ratio, microwave time, solid-liquid ratio, ultrasonic time and power, with Plackett-Burman （PB） experiment and jujube as a raw material. We further acquired the most obvious influential factor of ultrasonic time, the second significant factor of solid-liquid ratio, and the least significant factors of micro-wave time and ultrasonic power in designated scopes, with Box-Behnken （BB） experiment and response surface analysis. Its optimal extraction conditions are microwave power of 440 W, microwave time of 64 s, solid-liquid ratio of 1：8, ultrasonic time of 21min, ultrasonic power of 300 W, and ultrasonic temperature of 2.0 ℃. Extraction rate of cAMP is 90.83% under these conditions. The research result can provide a better natural active substance for pharmaceutical and food industries, and a reference for the further processing and industrialized applications of Ziziphus jujube and other relative products.