| 重组BbFGF工程菌的发酵条件 |
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| 引用本文： | 熊盛,林剑,姚汝华,刘杰森,张玲,张美英,李志英.重组BbFGF工程菌的发酵条件[J].华南理工大学学报(自然科学版),2001,29(4):53-58. |
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| 作者姓名： | 熊盛 林剑 姚汝华 刘杰森 张玲 张美英 李志英 |
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| 作者单位： | 1. 华南理工大学生物工程系,
2. 暨南大学生物工程研究所, |
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| 基金项目： | National Scientific and Technological Research Project for the Ninth Five_Year Plans |
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| 摘 要： | 利用大肠杆菌BL21（DE3）pLysS生产重组牛碱性成纤维细胞生长因子(BbFGF),对此工程菌的发酵条件进行了研究，在前期试验的基础上，重点探索培养基、诱导剂及区葡萄糖添加方式对T7启动子指导下的bFGF合成的影响，根据摇瓶试验和分批发酵试验结果，建立了一套变速加葡萄糖的高密度补料分批发酵工艺，在此工艺下，经11h发酵，可得光密度为30.7、bFGF产量为95mg/L的发酵产物，两步法纯化目的蛋白，得到纯度为95%的重组bFGF，其生物活性和标准品一致。
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| 关 键 词： | 大肠杆菌BL21（DE3）pLysS T7启动子 补料分批发酵 牛碱性成纤维细胞生长因子 工程菌 发酵工艺 培养基 BbFGF |
| 文章编号： | 1000-565X(2001)04-0053-06 |
Fermentation Conditions of Recombinant Bovine Fibroblast Growth Factor in Escherichia coli |
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| XIONG Sheng,Lin Jian,Yao Ru_hua,Liu Jie_sen,Zhang Ling,Zhang Mei_ying,Li Zhi_ying.Fermentation Conditions of Recombinant Bovine Fibroblast Growth Factor in Escherichia coli[J].Journal of South China University of Technology(Natural Science Edition),2001,29(4):53-58. |
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| Authors: | XIONG Sheng Lin Jian Yao Ru_hua Liu Jie_sen Zhang Ling Zhang Mei_ying Li Zhi_ying |
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| Abstract: | Key factors of the fermentation of a recombinant, E.coli BL21(DE3)pLysS producing basic fibroblast growth factor (bFGF) were studied. Flask_shaking experiments and batch fermentations were performed to investigate the effects of medium, inducer and glucose_feeding mode on bFGF formation directed by a T7 promoter. A fed_batch fermentation condition of high cell density with stepwise increased feeding of glucose was established. After undergoing fermentation for 11 h, the cell optical density was 30.7, and the amount of bFGF produced by E.coli reached 95 mg per liter culture. This target protein was purified to homogeneity (purity of 95%)from the supernatant of bacteria lysate and was found to be biologically identical to bFGF standard. |
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| Keywords: | bFGF E coli BL21(DE3)pLysS T7 promoter fed_batch fermentation |
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