| 烟草中游离糖类的BSTFA衍生化与气相色谱-质谱联用分析方法研究 |
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| 引用本文： | 周斌,张承明,张承聪,李茂昌.烟草中游离糖类的BSTFA衍生化与气相色谱-质谱联用分析方法研究[J].云南民族大学学报(自然科学版),2007,16(3):239-242. |
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| 作者姓名： | 周斌 张承明 张承聪 李茂昌 |
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| 作者单位： | 1. 云南大学化学科学与工程学院,云南昆明,650091
2. 云南省烟草科学研究院,云南昆明,650106 |
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| 摘 要： | 研究了衍生化试剂BSTFA对烟草中糖类的衍生化反应,并用GC-MS进行分析.烟草中的糖类用水超声提取,用C18SPE柱预分离,衍生化后用GC-MS分析测定.结果表明,该方法的检出限范围是4.7~7.6 ng,加标回收率为96%~106%,相对标准偏差(RSD)为4.63%~6.06%.实验证明该方法具有较高的准确度、快速和简便的特点.
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| 关 键 词： | 糖类 衍生化 |
| 文章编号： | 1672-8513（2007）03-0239-04 |
| 收稿时间： | 2007-04-02 |
| 修稿时间： | 2007-04-02 |
Analysis of Carbohydrates of the Tobacco as BSTFA Derivatives by GC-MS |
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| Zhou Bin,Zhang Chengming,Zhang Chengcong,LI Maochang.Analysis of Carbohydrates of the Tobacco as BSTFA Derivatives by GC-MS[J].Journal of Yunnan Nationalities University:Natural Sciences Edition,2007,16(3):239-242. |
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| Authors: | Zhou Bin Zhang Chengming Zhang Chengcong LI Maochang |
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| Institution: | 1. College of Chemistry Science and Engintering,Yunnan University, Kunming 650091, China; 2. Yunnan Academy of Tobacco Science,Kunming 650106,China |
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| Abstract: | The study researched the derivative way of carbohydrates with BSTFA by GC - MS. We used the way to analysis carbohydrates of tobacco. The carbohydrates of tobacco are extracted by the ultrasonic and beforehand separated by C18 SPE . The carbohydrates are derivatived by BSTFA and then determined by gas chromatogra- phymass spectrometry. The limits of detection of this method ranged from 4.7 - 7.6 ng. The recoveries of the method ranged from 96% - 106% and the relative standard deviations of peak areas ranged from 4.63% - 6.06%. Therefore, the results prove the way is precise,quick and convenient. |
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| Keywords: | BSTFA GC-MS |
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