毛细管区带电泳测定人工冬虫夏草菌丝中的有效成分

建立了毛细管区带电泳测定人工冬虫夏草菌丝中有效成分腺苷、腺嘌呤、尿苷的分析方法.采用15 mmol/L硼砂+14 mmol/L 磷酸二氢钠+体积分数5%甲醇(pH=9.50)作为缓冲体系,在电压为18 kV和检测波长为254 nm的条件下,人工冬虫夏草菌丝提取液中的有效成分实现了基线分离.定量分析表明,各有效成分的峰面积与质量浓度呈较好的线性关系(r≥ 0.998 5 ).此外,本实验还讨论了缓冲溶液的pH值、浓度及有机改性剂对有效成分迁移行为的影响.

Determination of the Active Components in Artificial Cordyceps by Capillary Zone Electrophoresis

An easy and low-cost method for the determination of the active components in artificial cordyceps by CZE was presented. Adenosine, adenine and uridine were baseline separated and quantitatively analyzed under the buffer conditions of 15 mmol/L disodium tetraborate + 14 mmol/L sodium dihydrogen phosphate + 5%(volum fraction) methanol (pH=9.50), applied voltage of 18 kV and detector wavelength of 254 nm. The linear relationship of the three components were good ( r ≥0.998 5 ). In addition, the effects of buffer pH value,concentration and organic modifier on the migration behavior of the solutes were also investigated.