幼龄和老龄SD大鼠肝、肾组织中生化指标变化的研究

目的研究过氧化脂质（LPO）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）在幼龄和老龄SD大鼠肝、肾组织中的变化，探讨此三种酶在机体衰老过程中的意义。方法21日龄和18月龄SD大鼠各10只，4℃取鼠肝、肾组织，用硫代巴比妥酸法测定LPO的含量；化学发光法测定SOD活力；DTNB直接法测定GSH—Px活力。结果幼龄SD大鼠肝、肾组织中LPO含量明显低于老龄鼠（P〈0．01）；SOD活力显著高于老龄鼠（P〈0．01）；GSH—Px的活力明显低于老龄鼠（P〈0．01）。结论LPO、SOD、GSH—Px在幼龄和老龄鼠中有显著的变化，对机体衰老过程的研究具有指标意义。

The Study of Variations of Biochemical Index in The Livers and Kidneys Tissues of Young and Aged SD Rats

Objective The variation of lipid peroxides （ LPO）, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） were tested in liver and kidney tissues of young and aged rats respectively; and to explore role of three enzymes in senescence process. Method There are ten twenty-one-day-SD rats and ten eighteen-month-SD rats. Liver and kidney tissues of rats were collected at 4℃; Thiobarbituric acid reaction was adopted to test LPO; Chemiluminescence used to test activity of SOD and activity of GSH-Px was tested through DTNB. Results Quantity of LPO in young rats＇ liver and kidney is significant lower compared to it in aged rats （P 〈 0.01 ） ; Activity of SOD is markedly higher in young rat group than that in aged group（ P 〈 0.01 ） ;Activity of GSH-Px was obviously lower in young rat group contrast to aged group. Conclusion There were significant variation of LPO, SOD and GSH-Px in liver and kidney issues between young rats and aged rats. These variations are meaningful for study of senescence process.