| 栝楼的根段及根尖培养与器官发生 |
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| 引用本文： | 陈惠.栝楼的根段及根尖培养与器官发生[J].山西师范大学学报,1994,8(1):53-57. |
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| 作者姓名： | 陈惠 |
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| 作者单位： | 山西师范大学生物系 |
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| 摘 要： | 离体培养栝楼的根段和根尖均能诱导产生不定芽.其发生部位在根尖端的0～3mm以内;最佳条件为:从茎尖或茎节段在含有NAAlmg/L的MS固体培养基上8d产生的不定根上截取长约0.5cm的根尖,接种在MS BA4mg/L的培养基上光下培养,可长出不定芽.
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| 关 键 词： | 栝楼 根段和根尖培养 器官发生 |
Organogenesis in Root Segments and Root Tips of Trichosanthes Kirilowii Maxin |
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| Chen Hui.Organogenesis in Root Segments and Root Tips of Trichosanthes Kirilowii Maxin[J].Journal of Shanxi Teachers University,1994,8(1):53-57. |
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| Authors: | Chen Hui |
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| Abstract: | Organogenesis duning the culture of root segents and root tips of Trichosan-thes kirilowii Maxin is reported. First, adventitions buds were induced in cultured excised root Segments and root tips, and the initiatedplace is on the root tips from 0 to 3 mm. Moreover, having Studied the factor of effecting adventious buds initiation and development. The best conidion of inducing adventitious buds is bellow: when shoot tip or stem node segments cultured on MS medium supplemented with NAA(1mg/L), after 8 days, adventitious root can be produced. Then cuting the root tips about 0. 5 cm and transferred to medium of MS BA4mg/L,on the conidition of culturing of lighting, 12 hours everyady after 15-30 days,adventitious buds can be induced and develooped. |
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| Keywords: | Trichosanthes kirilowii Maxin Root segments Root tips culture Adventitious bud Organogenesis |
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